ABSTRACT-The enrichment of bedding for vermicomposting systems with organic and inorganic additives is a technique which can promote the quality of produced vermicompost. Phosphate rock and fishmeal are two available and low-cost substances which can be provided easily and inoculation of vermicompost with phosphate solubilizing microorganisms (PSMs) can enhance the availability...

Amidophosphorihosyltransferase (EC 2.4.2.14) has been partially purified from pigeon liver, and ligand-induced alterations in molecular and kinetic properties have been studied. In Tris-HCl buffer the predominant form of’ the enzyme has an sZO,w of 5.9 * 0.7, Stokes radius of 42 A, and estimated molecular weight of 102,000. Incubation with phosphoribosylpyrophosphate (PP-ribose-P) results in an...

Here, we report a remarkable 15 Å nanolayered tin phosphate, Sn(HPO4)2·3H2O (SnP-H+ or SnP), and its clay-like gel, which are multifunctional and are prepared using earth-abundant Sn and P chemicals by a facile, environmentally benign and potentially cost-effective process. This new energy material is discovered here as the best proton conductor among all the known layered phosphates with a ver...

The mechanism by which the mammalian kidney excretes inorganic phosphate has not been fully elucidated. The only micropuncture data available are those of Walker and Hudson (1), and they deal with the mode of phosphate excretion in two amphibian species. These investigators reported that the inorganic phosphate concentration rises progressively along the proximal and distal nephron of the frog ...

Plastids of nongreen tissues import carbon as a source of biosynthetic pathways and energy. Within plastids, carbon can be used in the biosynthesis of starch or as a substrate for the oxidative pentose phosphate pathway, for example. We have used maize endosperm to purify a plastidic glucose 6-phosphate/phosphate translocator (GPT). The corresponding cDNA was isolated from maize endosperm as we...

A set of phosphatases was evaluated for their potential to catalyze the regioand stereoselective phosphorylation of alcohols using a high-energy inorganic phosphate donor, such as di-, triand polyphosphate. Parameters such as type and amount of phosphate donor and pH of the reaction were investigated in order to minimize the thermodynamically favored hydrolysis of the phosphate donor and the fo...

Reaction 1 occurring with reaction 2 involves carbon dioxide fixation. Koepsell & Johnson (1942) obtained a cell-free extract from Clo8tridium butylicum which converts pyruvic acid into acetic acid, carbon dioxide and hydrogen, and requires phosphate for the reaction. Later Koepsell, Johnson & Meek (1944) found that inorganic phosphate is taken up and labile phosphate appears. The overall react...

Sites of alkaline phosphatase activity have been located by an electron microscopic histochemical (Gomori) technique in vegetative cells of a repressible strain SB15 of Bacillus subtilis, derepressed and repressed by inorganic phosphate, and in a mutant SB1004 which forms alkaline phosphatase in a medium high in phosphate. The sites of enzyme activity were revealed as discrete, dense, and large...

Recent results suggest a paradigm shift from viewing inorganic phosphate as a passive requirement for basic cell functions to an active regulator of cell behavior. We have previously shown that elevated concentrations of phosphate increased cell proliferation and expression of protumorigenic genes such as Fra-1 and osteopontin in a preosteoblast cell line. Therefore, we hypothesized that elevat...

Several untested aspects of the regulation of inorganic nutrient uptake were examined using nutrient depletion experiments with the symbiotic coral Stylophora pistillata. The total inhibition of phosphate uptake in artificial seawater lacking sodium indicates the involvement of a sodium/phosphate symporter for the uptake of phosphate across host membranes. Addition of ammonium or nitrate (up to...