نتایج جستجو برای: infection process including spore germination

تعداد نتایج: 2542389  

Journal: :Journal of bacteriology 2012
Arturo Ramirez-Peralta Kerry-Ann V Stewart Stacy K Thomas Barbara Setlow Zhan Chen Yong-qing Li Peter Setlow

Bacillus subtilis isolates lacking the SpoVT protein, which regulates gene expression in developing forespores, gave spores that released their dipicolinic acid (DPA) via germinant receptor (GR)-dependent germination more rapidly than wild-type spores. Non-GR-dependent germination via dodecylamine was more rapid with spoVT spores, but germination via Ca-DPA was slower. The effects of a spoVT mu...

Journal: :Journal of bacteriology 1983
H Boschwitz Y Milner A Keynan H O Halvorson W Troll

The germination of Bacillus cereus T spore suspensions is partially prevented by several inhibitors of trypsin-like enzymes. Leupeptin, antipain, and tosyl-lysine-chloromethyl ketone are effective inhibitors, whereas chymostatin, elastatinal, and pepstatin are inactive. A synthetic substrate of trypsin, tosyl-arginine-methyl ester, also inhibits germination. Its inhibitory effect decreases as a...

Journal: :Virology & Immunology Journal 2017

Journal: :The EMBO journal 2005
Wilfried J J Meijer Virginia Castilla-Llorente Laurentino Villar Heath Murray Jeff Errington Margarita Salas

Phage phi29 is a virulent phage of Bacillus subtilis with no known lysogenic cycle. Indeed, lysis occurs rapidly following infection of vegetative cells. Here, we show that phi29 possesses a powerful strategy that enables it to adapt its infection strategy to the physiological conditions of the infected host to optimize its survival and proliferation. Thus, the lytic cycle is suppressed when th...

Journal: :Journal of bacteriology 2010
Pengfei Zhang Will Garner Xuan Yi Ji Yu Yong-qing Li Peter Setlow

The simultaneous nutrient germination of hundreds of individual wild-type spores of three Bacillus species and a number of Bacillus subtilis strains has been measured by two new methods, and rates of release of the great majority of the large pool of dipicolinic acid (DPA) from individual spores of B. subtilis strains has been measured by Raman spectroscopy with laser tweezers. The results from...

2014
Yan Chen W. Keith Ray Richard F. Helm Stephen B. Melville David L. Popham

Bacterial endospores exhibit extreme resistance to most conditions that rapidly kill other life forms, remaining viable in this dormant state for centuries or longer. While the majority of Bacillus subtilis dormant spores germinate rapidly in response to nutrient germinants, a small subpopulation termed superdormant spores are resistant to germination, potentially evading antibiotic and/or deco...

Journal: :Journal of bacteriology 2010
Jared D Heffron Emily A Lambert Nora Sherry David L Popham

Bacterial spores remain dormant and highly resistant to environmental stress until they germinate. Completion of germination requires the degradation of spore cortex peptidoglycan by germination-specific lytic enzymes (GSLEs). Bacillus anthracis has four GSLEs: CwlJ1, CwlJ2, SleB, and SleL. In this study, the cooperative action of all four GSLEs in vivo was investigated by combining in-frame de...

Journal: :Journal of bacteriology 1973
A Hey-Ferguson M Mitchell A D Elbein

Evidence is presented which indicates that utilization of trehalose is an early event in spore germination in Streptomyces hygroscopicus. Early in spore germination and before any increase in cell mass, the activity of trehalase increased more than 15-fold, while the intracellular content of trehalose fell to very low levels. On the other hand, increased activity of the trehalose phosphate synt...

Journal: :Applied and environmental microbiology 2002
Madan Paidhungat Barbara Setlow William B Daniels Dallas Hoover Efstathia Papafragkou Peter Setlow

Spores of Bacillus subtilis lacking all germinant receptors germinate >500-fold slower than wild-type spores in nutrients and were not induced to germinate by a pressure of 100 MPa. However, a pressure of 550 MPa induced germination of spores lacking all germinant receptors as well as of receptorless spores lacking either of the two lytic enzymes essential for cortex hydrolysis during germinati...

2005
M. L. FIELDS N. FINLEY

FIELDS, AI. L. (University of MIissouri, Columbia), AND N. FINLEY. Effect of carbohydrates in phosphate buffer on germination of Bacillus stearothermophilus spores. Appl. l\Iicrobiol. 11: 453-457. 1963.-Among strains, and among spore suspensions of the same strain, different spore-germination responses were observed when spores were heated in monosaccharides, disaccharides, and polysaccharides ...

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