Besides the high starch content, sweet potato contains endogenous amylases where the two Predominants are - and β-amylases. Sweet potato is thought to be a promising source of β -amylase since β -amylase is one of the major protein in the tubers. This investigation has focused on the properties of β amylase from white-flesh sweet potato that has been grown in Iran as a potential source for indu...

BACKGROUND We previously cloned a 1,3-specific lipase gene from the fungus Rhizomucor miehei and expressed it in methylotrophic yeast Pichia pastoris strain GS115. The enzyme produced (termed RML) was able to catalyze methanolysis of soybean oil and showed strong position specificity. However, the enzyme activity and amount of enzyme produced were not adequate for industrial application. Our go...

alpha-amylase is widely used as an industrial enzyme, and a therapeutic target for which inhibitors are designed. organic solvents are used to dissolve various compounds that would be studied as moderators of alpha-amylases, but they could themselves affect enzyme activity and stability. methanol, ethanol and propanol are simple alcohols that may be commonly used to this end, and their effect h...

The objective of this work is investigation of the adsorption and decomposition of Reactive Blue 19 from industrial wastewaters using laccase enzyme immobilized on activated carbon powder and fiber as adsorbent. Time, pH, temperature, stirring rate, the amount of the adsorbent, dye initial concentration, solution flow rate in the column and column height were studied as key operating parame...

Lipases are enzymes of immense industrial relevance, and, therefore, are being intensely investigated. In an attempt to characterize lipases at molecular level from novel sources, a lipase gene from Bacillus amyloliquefaciens PS35 was cloned, heterologously expressed in Escherichia coli DH5α cells and sequenced. It showed up to 98% homology with other lipase sequences in the NCBI database. The ...

The demand for enzymes in the global market is projected to rise at a fast pace in recent years. There has been a great increase in industrial applications of pectinase owing to their significant biotechnological uses. For applying enzymes at industrial scale primary it is important to know the features of the enzyme. Thus, this study was undertaken with aims of characterizing the pectinase enz...

The present study describes the screening of different agroindustrial byproducts for enhanced production of alkaline protease by a wild and EMS induced mutant strain of Bacillus subtilis IH-72(EMS8). During submerged fermentation, different agro-industrial byproducts were tested which include defatted seed meals of rape, guar, sunflower, gluten, cotton, soybean, and gram. In addition to these m...

Enzymes found in nature have been exploited in industry due to their inherent catalytic properties in complex chemical processes under mild experimental and environmental conditions. The desired industrial goal is often difficult to achieve using the native form of the enzyme. Recent developments in protein engineering have revolutionized the development of commercially available enzymes into ...

The renewed interest in exploring various natural habitat/environments for discovering novel microbial sources as stable cholesterol oxidase producers is on the incline due to its broad-range of clinical and industrial applications. A search was conducted to isolate cholesterol oxidase producing bacteria from samples collected from different environments. A total of 98 bacterial isolates capabl...

The present paper addresses two crucial features in the industrial development of fructose production by enzymatic hydrolysis of inulin: the use of immobilized biocatalyst in the hydrolysis of crude extracts of chicory roots and the evaluation of the effect of degree of polymerization of inulin on the overall reaction rate. The immobilized biocatalyst consisted of inulinase covalently bound to ...