An efficient procedure has been researched for the solvent-free synthesis of bis(indolyl)methanes via a one-pot reaction of indoles and aldehydes or ketones promoted by PEG1000-based dicationic acidic ionic liquid (PEG1000-DAIL). The catalyst PEG1000-DAIL could be reused seven times with excellent results. Furthermore, through this method, a highly chemoselective reaction of benzaldehyde and ac...

In the lipophilic extracts from Streptomyces cinnamomeus 2-ethyl-5-(3-indolyl)oxazole (1a) was detected by chemical screening methods. The structure of the crystalline 1a was determined by spectroscopic and X-ray analysis. The new mono- and dibromo derivatives 1b and 1c are described. 1a is identical with pimprinethine and belongs to a group of microbial indole alkaloids, which can be regarded ...

Ammonium monovanadate (NH4VO3) has been devoted as an efficient, commercially available, eco-friendly and reusable catalyst for the synthesis of bis(indolyl)methanes (BIMs), oxindole derivatives and also Michael adducts of indoles at 50 °C under solvent-free conditions. The reusability of this solid acid catalyst in addition with its selectivity has also been examined.

A shuttle vector designated pMAD was constructed for quickly generating gene inactivation mutants in naturally nontransformable gram-positive bacteria. This vector allows, on X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) plates, a quick colorimetric blue-white discrimination of bacteria which have lost the plasmid, greatly facilitating clone identification during mutagenesis. The ...

The mol-ecule of the title compound, C(17)H(14)N(4)O(4), uses its amide -NH- group to form a hydrogen bond to the amido -C(=O)- group of an adjacent mol-ecule to furnish a linear chain structure. The hydr-oxy group forms an intra-molecular hydrogen bond; the indolyl -NH- unit does not engage in any strong hydrogen-bonding inter-actions.

The indolyl -NH group of the title Schiff base, C(19)H(19)N(3)O(2), forms a hydrogen bond to the -OH group of an inversion-related mol-ecule, resulting in a hydrogen-bonded dimer; adjacent dimers are further linked through an inter-dimer N-H⋯O hydrogen bond involving the -C(=O)-NH-N=fragment to form a linear ribbon that runs along the a axis.

In situ hybridization techniques typically employ chromogenic staining by enzymatic amplification to detect domains of gene expression. We demonstrate the previously unreported near infrared (NIR) fluorescence of the dark purple stain formed from the commonly used chromogens, nitro blue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP). The solid reaction product has significant...

Cytochrome P450 CYP71A13 of Arabidopsis lyrata is a heme protein involved in biosynthesis of indole-3-acetonitrile which leads to the formation of indolyl-3-acetic acid. It catalyzes a unique reaction: formation of a carbon-nitrogen triple bond and dehydration of indolyl-3-acetaldoxime. Homology model of this 57 kDa polypeptide revealed that the heme existed between H-helix and J- helix in the ...

Two spin-labelled derivatives of the 5-(2-indolyl)-2,4-pentadienoyl class of inhibitors of the vacuolar ATPase have been synthesised and their EPR properties characterised in phospholipid membranes. One spin-labelled inhibitor is the amide derivative of pentadienic acid and 4-amino-TEMPO (INDOL6), and the other is the 3-hydroxymethyl-PROXYL ester (INDOL5). The response of the EPR spectra to the...