The mean increase in activation of the MgZ+-ATPase was 2.95f1.23 (n = 5 ) for muscle myosin and 2.90 for the platelet myosin and the most favourable combining ratios (w/w) for both lay between 2:l and 4:l actin to myosin. An antibody has been raised to the leucocyte actin, and we have used this for the detection of actin filaments in a variety of cells, including guinea-pig polymorphonuclear le...

AIMS To investigate whether autoantibodies to choroid plexus are present in human senile dementia. METHODS Serum samples from 40 elderly people presenting with characteristic, diagnostic criteria of senile dementia of Alzheimer's type and 20 age matched healthy controls were tested by indirect immunofluorescence for the presence of autoantibodies to choroid plexuses, using frozen sections of ...

The reactivities of Ca1 and HMFG2 monoclonal antibodies were compared on paraffin wax embedded breast tissues using indirect immunoperoxidase. The expression of Ca antigen, like HMFG2, is not exclusive to malignancy: Ca was present in 41/53 (77%) and HMFG2 in 42/53 (79.2%) non-malignant conditions and both were present in 33/35 (94%) carcinomas. Similar results were obtained when cryostat secti...

Mice infected with Trypanosoma cruzi, but parasitologically cured after specific chemotherapy, continued to exhibit positive indirect immunofluorescence serological tests 3-6 months after the therapy. Treatment of trypanosome antigens with monospecific antisera produced in rabbits, and examination by immunoelectron-microscopy following peroxidase labelling disclosed the presence of membrane dep...