Tachyzoite forms of Toxoplasma gondii were subjected to a sequential organic solvent extraction, which allows fractionation of membrane components according to their degrees of hydrophobicity, yielding three fractions named F1 (most hydrophobic) to F3 (least hydrophobic). Fractions F2 (80.85% specificity and 86.95% sensitivity) and F3 (89.36% specificity and 93.61% sensitivity) gave the best re...

We previously established a cell line from a patient with acute myelomonocytic leukemia with eosinophilia (M4E0), ME-1. ME-1 cells are responsive to colony-stimulating factors (CSFs) such as interleukin-3 (IL-3), IL-4, and granulocyte-macrophage CSF (GM-CSF), and exhibit monocyte-macrophage differentiation. We isolated three subclones, ME-F1 from ME-1, and ME-F2 and ME-F3 from two sublines of M...

Let n and r be positive integers. Suppose that a family F ⊂ 2[n] satisfies |F1 ∩ F2 ∩ F3| ≥ 2 for all F1, F2, F3 ∈ F . We prove that if w < 0.5018 then ∑ F∈F w |F |(1− w)n−|F | ≤ w2.

A sampling design method that seeks chlorine sampling locations using a multiobjective optimization framework is proposed here. The design problem is formulated as a threeand two-objective optimization where the parameter estimation accuracy (F1) is maximized, the the number of sensors is minimized (F2) and the weighted nodal sensitivity is maximized (F3). Trade-off curves are generated using t...

BACKGROUND The aim of this study was to develop a method to separate and quantify subforms of free prostate-specific antigen (fPSA) in serum by two-dimensional electrophoresis and to assess the diagnostic accuracy of these subforms for prostate cancer (PCa) diagnosis in comparison with total PSA (tPSA) and the ratio of fPSA to tPSA (%fPSA). METHODS Sera from 50 patients with and without PCa, ...

Let t ≥ 26 and let F be a k-uniform hypergraph on n vertices. Suppose that |F1∩F2∩F3| ≥ t holds for all F1,F2,F3 ∈F . We prove that the size of F is at most (n−t k−t ) if p = k n satisfies p≤ 2 √ 4t +9−1 and n is sufficiently large. The above inequality for p is best possible.

Chemical forms of Zn, Ni, Cu, and Pb in municipal sewage sludge were investigated by adding humus soil to sludge and by performing sequential extraction procedures. In the final sludge mixtures, Zn and Ni were mainly found in Fe/Mn oxide-bound (F3) and organic matter/sulfide-bound (F4) forms. For Zn, exchangeable (F1), carbonate-bound (F2), and F3 forms were transformed to F4 and residual forms...

Mahamrutyunjaya rasa (MHR), an Ayurvedic formulation, used as cardiotonic, contains potentially toxic compounds like aconitine, which are detoxified during preparation using traditional methods. Comparative toxicological evaluation of laboratory prepared formulation (F1) and two marketed formulations (F2 and F3) were performed based on their effects on viability of H9c2 cells and after single o...

Classical Mendelian experiments were conducted to determine the genetics and inheritance of quinclorac and acetolactate synthase (ALS)-inhibitor resistance in a biotype of Galium spurium. Plants were screened with the formulated product of either quinclorac or the ALS-inhibitor, thifensulfuron, at the field dose of 125 or 6 g active ingredient (a.i.) ha respectively. Segregation in the F2 gener...