A December 2014 meeting reviewed Ebola virus immunology relevant to vaccine development, including Ebola prevention, immunity, assay standardization, and regulatory considerations. Vaccinated humans appear to achieve immune responses comparable in magnitude with those associated with protection in nonhuman primates, suggesting that immunological data could be used to demonstrate vaccine efficacy.

BACKGROUND IFN-γ is presently the only soluble immunological marker used to help diagnose latent Mycobacterium tuberculosis (M.tb) infection. However, IFN-γ is not available to distinguish latent from active TB infection. Moreover, extrapulmonary tuberculosis, such as tuberculous pleurisy, cannot be properly diagnosed by IFN-γ release assay. As a result, other disease- or infection-related immu...

We combine total internal reflection fluorescence structured illumination microscopy with spatiotemporal image correlation spectroscopy to quantify the flow velocities and directionality of filamentous-actin at the T cell immunological synapse. These techniques demonstrate it is possible to image retrograde flow of filamentous-actin at superresolution and provide flow quantification in the form...

PURPOSE The objectives of this study were to assess the toxicity and immunological response induced by the intradermal (i.d) administration of tumor lysate-pulsed dendritic cells (DCs). EXPERIMENTAL DESIGN Patients with stage IV solid malignancies were treated in cohorts that received 10(6), 10(7), and 10(8) DCs i.d. every 2 weeks for three vaccines. Each vaccine was composed of a mixture of ...

The COVID-19 pandemic has exposed the extent of global connectivity and collective vulnerability to emerging diseases. From its suspected origins in Wuhan, China, it spread all corners world a matter months. absence high-performance, rapid diagnostic methods that could identify asymptomatic carriers contributed worldwide transmission. Serological tests offer numerous benefits compared other ass...

A ferromagnetic gold nanoparticle based immune detection assay, exploiting the enhanced signal amplification of inorganic nanozymes, was developed and evaluated for its potential application in Mycobacterium tuberculosis complex (MTBC) organisms, simultaneous identification bovis. Ferromagnetic nanoparticles (Au–Fe3O4 NPs) were prepared their intrinsic peroxidase-like activity exploited to cata...

Background: Toxocara infection is one of the most neglected tropical diseases that has remained undiagnosed in developing countries. The disease leads to many signs and symptoms such as fever, coughing or wheezing, belly pain, hepatomegaly, loss appetite, rash, lymphadenopathy, red painful eyes, strabismus, blurred cloudy vision, like. Objective: present study aimed at investigating seroprevale...