This report summarizes an evaluation of the fully automated Olympus Hite System (AHS, Olympus, D-2000 Hamburg) for serum protein fractionation. The precision thereof exceeded that of the semi-manually processed Multiphorese System II MS 755 (Vogel, D-6300 Giessen). It proved to be rapid and easy to maintain. A comparison of the albumin values obtained by AHS and Beckman immunochemistry system s...

During the course of growth of Arthrobacter oxidans, induction of the enantiozymes 6-hydroxy-D-nicotine oxidase and 6-hydroxy-L-nicotine oxidase occurred in the presence of DL-nicotine. Cryoultramicrotomed sections obtained from cells grown to stationary phase were gold immunolabeled. The results obtained demonstrate that both enzymes are localized in the cytoplasm.

1. Dept. Physiology, University of Toronto, 1 King’s College Circle, Toronto, ON, Canada M5S 1A8. 2. Instituto Butantan, Lab. of Pharmacology, Biochemistry and Immunochemistry, respectively, São Paulo, SP, Brazil. 3. Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON, Canada M5G 1X5. 4. Current address: Department of Biological Science, Barnard College...

Primary ductal adenocarcinoma is a rare subtype of adenocarcinoma. Literature review showed 13 cases of primary ductal adenocarcinoma of the lacrimal gland. We report a case of primary ductal adenocarcinoma of the lacrimal gland and review of literature highlighting its clinical presentation, histopathology including immunochemistry and overall outcome.

In rats, a shift from somatotroph dominance to lactotroph dominance during pregnancy and lactation is well reported. Somatotroph to lactotroph transdifferentiation and increased lactotroph mitotic activity are believed to account for this and associated pituitary hypertrophy. A combination of cell death and transdifferentiation away from the lactotroph phenotype has been reported to restore non...

A case of mixed acute leukaemia with T lymphoblastic, myeloblastic, and monocytic components is described. The use of immunological markers, ultrastructural morphology, cytochemistry, immunochemistry, and combined techniques, simultaneously detecting two markers in individual cells, made it possible to define the different blast cell populations.

Pertinent issues of collagen antigenicity and immunogenicity are concisely reviewed as they relate to the design and application of biomedical devices. A brief discussion of the fundamental concepts of collagen immunochemistry is presented, with a subsequent review of documented clinical responses to devices containing reconstituted soluble or solubilized collagen. The significance of atelocoll...

Jay P. Shah, Terry M. Phillips, Jerome V. Danoff, and Lynn H. Gerber Rehabilitation Medicine Department, Clinical Research Center, National Institutes of Health; Ultramicro Analytical Immunochemistry Resource, Division of Bioengineering and Physical Science, Office of Research Services, National Institutes of Health, Bethesda, Maryland; and Department of Exercise Science, George Washington Univ...

The new trends in immunochemistry related to the replacement of radioisotopic labels with non-radioactive labels are presented. Immunoenzymatic, fluorescent and chemiluminescent techniques are described in terms of their basic principles and their most common applications. The advantages of computer-controlled calibration are also discussed.