نتایج جستجو برای: immunoblotting
تعداد نتایج: 12257 فیلتر نتایج به سال:
The type I collagen was studied in samples of two types osteoplastic materials produced the Biotech Research Institute Samara State Medical University using immunoblotting. demineralized used work were compact bone powder and crushed material human cancellous tissue. Collagen its polypeptides separated a 5% polyacrylamide gel with 3.6 M urea according to method Hayashi Nagai (1979). advantage i...
Abstract Background Cholangiocarcinoma represents the second most common primary liver malignancy. The incidence rate has constantly increased over last decades. silent nature limits early diagnosis and prevents efficient treatment. Methods Immunoblotting immunohistochemistry were used to assess expression profiling of USP9X EGLN3 in cholangiocarcinoma patients. ShRNA was silence gene expressio...
the importance of amaranthus retroflexus pollen in causing respiratory allergy has been well ascertained in many countries including iran with a high positive rate (69%) among iranian allergic patients. the aim of the present study is to identify the allergenic properties of a. retroflexus pollen. sixteen patients with allergy to a. retroflexus pollen were selected for the study. the antigenic ...
Two new methods for typing Clostridium difficile, immunoblotting and plasmid fingerprinting, were compared with serotyping and polyacrylamide gel electrophoresis (PAGE). Of these methods, immunoblotting was found to be the most valuable for use in a comprehensive typing system. More groups could be distinguished by immunoblotting than by serotyping or PAGE. Immunoblotting results were also more...
Present: Due to an error made during the final figure assembly, blots for U266 were mistakenly used in lieu of blots for JJN3 in Figure 2A.. overnight followed by cell lysate preparation and immunoblotting analysis for p-STAT3 (Tyr705) and total STAT3. B. OPM2 cells were treated with SC99 at the indicated concentrations followed by the analysis of the expression of p-STAT3. C. OPM2 cells were t...
We developed a rapid, accurate method for phenotyping apoprotein E that can be used for large-scale population studies. In this method, adapted from the method of Kamboh et al. (J Lipid Res 1988;29:1535-43), 10-microL plasma samples are incubated with dithiothreitol and Tween-20 for 15 min and then applied to 5% polyacrylamide gels containing ampholyte (pH 4.5-8) and urea (3 mol/L). After 2 h o...
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