The in vitro developmental competence of equine oocytes is still low in comparison with other domestic mammals. A major factor affecting the viability of cells during in vitro culture is the increased oxidative stress. Oxidative modifications could be responsible for oocyte incompetence for in vitro maturation (IVM). Cysteamine, a glutathione (GSH) synthesis enhancer, has been shown to increase...

abstract: introduction & objective: the infertility treatment by using in vitro matured oocyte has many potential applications. it minimizes the risk of ovarian hyper stimulation and is an alternative treatment for women with polycystic ovarian syndrome who may have problems regarding stimulation for ivf. this method may prove important for subjects in need of fertility preservation and also pr...

Porcine immature oocyte quality (i.e., that of live oocytes at the germinal vesicle stage) was evaluated according to features of the surrounding cumulus, aiming to establish maturational competence of different subpopulations of such cumulus-oocyte complexes. Six subpopulations were identified: A1 (with a dense cumulus), A2 (with a translucent cumulus), B1 (with the corona radiata), B2 (partly...

We investigated the effects of vitamin B1 deficiency on the meiosis maturation of oocytes. Female Crl:CD1 (ICR) mice were fed a 20% casein diet (control group) or a vitamin B1-free diet (test group). The vitamin B1 concentration in ovary was approximately 30% lower in the test group than in the control group. Oocyte meiosis was not affected by vitamin B1 deficiency when the deficiency was not a...

Previous cases reported recurrent all oocytes in repeated cycles were arrested at MI failed to mature after culture and ICSI resulted total nonfertilization. Ionomycin induced first PB extrusion patients with maturation-resistant oocytes. combined assisted oocyte activation (AOA) on MII matured vitro abnormal fertilization no further embryo cleavage potential. The objective of this work was res...

BACKGROUND Although anandamide (AEA) had been measured in human follicular fluid and is suggested to play a role in ovarian follicle and oocyte maturity, its exact source and role in the human ovary remains unclear. METHODS AND FINDINGS Immunohistochemical examination of normal human ovaries indicated that the endocannabinoid system was present and widely expressed in the ovarian medulla and ...

Fertilization of the immature, prophase I-arrested mouse oocyte produces multiple Ca2+ transients similar to those of the mature, metaphase II egg; however, the first Ca2+ transient is much lower in amplitude and shorter in duration. In contrast to prophase I-arrested oocytes, maturing oocytes fertilized after germinal vesicle breakdown have first Ca2+ transients similar to those of mature fert...

Assisted reproductive techniques such as oocyte transfer and intracytoplasmic sperm injection (ICSI) can be used clinically to produce foals from mares with reproductive abnormalities that prevent normal breeding or production of embryos. ICSI can also be used to produce foals from stallions with poor fertility or from semen with limited quantity. Both of these procedures require the collection...