Diploid and continuous cell lines are used to propagate viral vaccines. At Human Viral Vaccine Department of Razi Vaccine and Serum Research Institute, MRC5 diploid cell is used for the development of live attenuated measles, mumps, rubella, and three types of poliovirus vaccines.  Additionally, three continuous cell lines (i.e., RK13, HeLa, and Vero) are applied in quality control tests. Accor...

In this paper, a serological assay for the detection of antibodies to Capillaria hepatica, a zoonotic parasite, is described. In the past, the only way of detecting Capillaria hepatica was to perform a liver biopsy. The indirect immunofluorescence (IIF) assay, based on liver sections of naturally infected mice and human serum samples, is suitable for detecting early stages of human infections a...

The serum-neutralization (SN) and the indirect-immunofluorescence (IIF) assays have invariably been used for detecting antibodies against bovine virus diarrhoea virus (BVDV) in cattle sera. An enzyme-linked immunosorbent assay (ELISA) was applied which has a sensitivity comparable with the SN and IIF in detecting antibody to BVDV. A total of 472 bovine sera were assayed and a high prevalence of...

Letter An increase in the segmentation visualization techniques resulted in a parallel, more frequent determination of complex renal cysts. Renal cysts were classified by Bosniak, and this classification was modified in 2003, in order to attempt to narrow the large interval between type II and type III by the definition of IIF category [1]. However, none of the recent diagnostic modalities coul...

Systemic sclerosis (SSc) is associated, in nearly all patients, with autoantibodies (Ab). Accordingly, and order to identify major (anti-CEN A/B anti-Topo I) but also minor Abs, the usefulness of combining indirect immunofluorescence (IIF) on HEp-2 cells an 11 multi-antigenic SSc immunodot was explored. 1689 samples tested at request clinicians, were evaluated retrospectively. The positivity ra...

We prove results that enable realization of heteroclinic networks in coupled homogeneous and heterogeneous systems of identical cells. We also consider various models for network dynamics that allow variation in the number of inputs and identical cells. Date: January 11, 2015. 1991 Mathematics Subject Classification. 37A250. Research supported in part by NSF Grant DMS-1265253 & Marie Curie IIF ...

BACKGROUND Detection of autoantibodies giving nuclear rim pattern by immunofluorescence (anti-nuclear envelope antibodies - ANEA) in sera from patients with primary biliary cirrhosis (PBC) is a useful tool for the diagnosis and prognosis of the disease. Differences in the prevalence of ANEA in PBC sera so far reported have been attributed to the methodology used for the detection as well as to ...

OBJECTIVE To 1) determine, using contemporary recombinant antigen-based assays, the aquaporin-4 (AQP4)-immunoglobulin G (IgG) detection rate in sequential sera of patients assigned a clinical diagnosis of neuromyelitis optica (NMO) but initially scored negative by tissue-based indirect immunofluorescence (IIF) assay; and 2) evaluate the impact of serostatus on phenotype and outcome. METHODS F...

PURPOSE To assess the clinical reliability of the Bosniak IIF category and to determine the proper radiologic follow-up duration and intervals for category IIF complex renal cysts. MATERIALS AND METHODS We studied 201 patients with category IIF renal cysts from January 1996 to January 2011. Renal cyst progression to category III was defined as an increase in complexity of the cyst in follow-u...

Anomaly detection in hyperspectral image (HSI) is affected by redundant bands and the limited utilization capacity of spectral–spatial information. In this article, we propose a novel improved Isolation Forest (IIF) algorithm based on assumption that anomaly pixels are more susceptible to isolation than background pixels. The proposed IIF modified version (iForest) algorithm, which addre...