Insulin-like growth factors (IGF) promote follicle development, ovulation, and embryonic development affect placental fetal in many mammalian species. Therefore, this study aimed to determine the relationship between birth type, plasma concentration, mRNA expression of IGF-I Bafra ewes. A total 30 ewes, which have single (n=15) or twin gestation at least gave 2, been used as experimental animal...

OBJECTIVE IGF-1 and the IGF-1 receptor (IGF-1R) have been implicated in the regulation of adipocyte differentiation and lipid accumulation in vitro. RESEARCH DESIGN AND METHODS To investigate the role of IGF-1 receptor in vivo, we have inactivated the Igf-1r gene in adipose tissue (IGF-1R(aP2Cre) mice) using conditional gene targeting strategies. RESULTS Conditional IGF-1R inactivation resu...

The effects of several human and chicken insulin-like growth factor (IGF) analogs on turkey satellite cell and embryonic myoblast proliferation were examined in serum-free medium. Similar rates of proliferation were observed when human or chicken IGF-I or IGF-II (13.1 nM) was administered to satellite cells. The biopotency of two analogs, which were modified to prevent interaction with IGF-bind...

An efficient expression system in Escherichia coli for several biologically active insulin-like growth factor-I (IGF-I) fusion peptide analogues is described. These novel IGF-I fusion protein analogues have properties that make them very useful reagents in the investigation of IGF-I action. The analogues comprise an IGF-I sequence and the first 11 amino acids of methionyl porcine growth hormone...

Conflicting data on the role of metabolic syndrome (MS) in development hepatocellular carcinoma (HCC) require studies influence molecular factors that are important HCC MS, which was goal our review. Publications (scientific articles and reviews) over past 10 years were studied analyzed using databases Web Science, Scopus, PubMed, RSCI. The terms used for search “metabolic non-alcoholic fatty l...

The effect of human growth hormone (GH) and insulin-like growth factor-l (IGF-l), IGF-ll, and insulin on human plasma cell responses was studied. GH enhanced lg production and thymidine uptake in the human plasma cell lines, IM-9 and AF-10. IGF-l, but not IGF-ll or insulin, also enhanced lg production and proliferation in them. However, enhancement by GH was not mediated by IGF-I, because enhan...

In a prior report we presented evidence that insulinlike growth factor-I (IGF-I) can act in an autocrine fashion by demonstrating that FRTL-5 cells transfected with hIGF-IA fusion genes express and secrete biologically active IGF-I that renders the stimulation of DNA synthesis in FRTL-5 cells independent of heir requirement for exogenous IGFs or insulin. To determine if IGF-1’s autocrine action...

Muscle is an important target tissue for insulin-like growth factor (IGF) action. The presence of specific, high affinity IGF receptors, as well as the expression of IGF peptides and binding proteins by muscle suggest that a significant component of IGF action in this tissue is mediated through autocrine and/or paracrine mechanisms. To explore autocrine/paracrine action of IGFs in muscle, we st...