Trypanosoma cruzi undergoes a biphasic life cycle that consists of four alternate developmental stages. In vitro conditions to obtain a synchronic transformation and efficient rates of pure intermediate forms (IFs), which are indispensable for further biochemical, biological, and molecular studies, have not been reported. In the present study, we established an improved method to obtain IFs fro...

Posttranslationally modified forms of tubulin accumulate in the subset of stabilized microtubules (MTs) in cells but are not themselves involved in generating MT stability. We showed previously that stabilized, detyrosinated (Glu) MTs function to localize vimentin intermediate filaments (IFs) in fibroblasts. To determine whether tubulin detyrosination or MT stability is the critical element in ...

BACKGROUND To establish appropriate measures that deal with incidental findings (IFs), the neuroscience community needs to address various ethical issues. The current state of research facilities regarding IFs and investigator attitudes as well as potentially eligible research participants must be assessed prior to future discussions and before the development of policies and guidelines. To thi...

OBJECTIVE Investigate the utility of intraoperative frozen section (iFS) in patients with follicular thyroid lesions following publication of the 2015 American Thyroid Association (ATA) guidelines. STUDY DESIGN Retrospective chart review. METHODS Patient demographics, preoperative cytology, frozen pathology, and final pathology were reviewed on patients undergoing thyroid surgery at a terti...

Increased activity of the noradrenergic system in the amygdala has been suggested to contribute to the hyperarousal symptoms associated with post-traumatic stress disorder (PTSD). However, only two studies have examined the content of noradrenaline or its metabolites in the amygdala of rats previously exposed to traumatic stress showing inconsistent results. The aim of this study was to investi...

Abstract We apply insights from the political economy of secession to analyse early years Irish Free State (IFS). The IFS was fortuitous in a debt settlement that enabled it begin its existence free while also receiving financial assistance quell civil unrest. Yet unable continue provide welfare spending inherited old regime thereby exacerbating inequality. maintained sterling peg, which led mi...

The Ca2+-modulated, dimeric proteins of the EF-hand (helix-loop-helix) type, S100A1 and S100B, that have been shown to inhibit microtubule (MT) protein assembly and to promote MT disassembly, interact with the type III intermediate filament (IF) subunits, desmin and glial fibrillary acidic protein (GFAP), with a stoichiometry of 2 mol of IF subunit/mol of S100A1 or S100B dimer and an affinity o...

The animal cell cytoskeleton consists of three interconnected filament systems: actin-containing microfilaments (MFs), microtubules (MTs), and the lesser known intermediate filaments (IFs). All IF proteins share a common tripartite domain structure and the ability to assemble into 8-12 nm wide filaments. Electron microscopy data suggest that IFs are built according to a completely different pla...

The geometry of fractals is rich enough that they have extensively been used to model natural phenomena and images. Iterated function systems (IFS) theory provides a convenient way to describe and classify deterministic fractals in the form of a recursive definition. As a result, it is conceivable to develop image representation schemes based on the IFS parameters that correspond to a given fra...

A fast coding algorithm for images using vector quantization (VQ) and pixelwise fractal approximation is proposed. The low frequency component of an input image is approximated and its residual is used to calculate the scaling factor of fractal transform. The scaling factor is compressed by transform VQ (TVQ). In the proposed method, lo encode a digital image by an iterated function system (IFS...