We examine the self-consistency of laboratory and observational data for potential carriers 3.3 $\mu$m infrared emission feature (IEF), a member ubiquitous family strong interstellar IEFs at 3.3, 3.4, 6.2, 7.7, 8.6, 11.2, 12.7 $\mu$m. Previous studies have shown that most Galactic sources (reflection nebulae, HII regions, planetary nebulae) show displaying similar central wavelengths, full widt...

Objectives: First, we sought to assess the frequency of incidental extra-cardiac findings (IEF) found in a consecutive series of clinical Cardiac MR scans. Second, we compared the 3 clinically used HASTE acquisition protocols in this context. Third, we determined the impact of the three different HASTE protocols on acquisition time and image quality. Methods: Three consecutive groups of 238 pat...

Stylar proteins of 13 almond (Prunus dulcis) cultivars with known S-genotypes were surveyed by IEF and 2D-PAGE combined with immunoblot and N-terminal amino acid sequence analyses to identify S-RNases associated with gametophytic self-incompatibility (SI) in this plant species. RNase activities corresponding to Sa and Sb, two of the four S-alleles tested, were identified by IEF and RNase activi...

To the Editor: In a recent article in your journal, Delanghe et al. [1]. reviewed both the indirect and direct methods for the detection of recombinant human erythropoietin (rhEPO) in doping analysis. However, some of the statements made are incorrect and some others deserve clarification. The direct detection of the misuse of rhEPO and analogues in sports is currently done in World Antidoping ...

The effects of mobility corrections on carrier ampholytes are studied at various ampholyte concentrations to understand protein behavior during IEF. IEF simulations are conducted in the presence of 25 biprotic carrier ampholytes within a pH range of 6-9 after applying the Onsager-Debye-Hückel correction to the carrier ampholytes. Two model proteins with ten charge states but without ionic stren...

A new immunoaffinity technique for purifying anti-apolipoprotein CIII antibodies has been developed by isolating bands of apo CIII2 from isoelectric focusing (IEF) gel. Total apo C was obtained from delipidated very-low-density lipoprotein by chromatography on Sephacryl S 200. Apo CIII2 was separated from the apo CII band by IEF on polyacrylamide gel with use of pH 4-6 ampholytes. After sonicat...

IEF can be used to differentiate human urinary erythropoietin (uEPO), recombinant human erythropoietin or epoetin (rEPO) and darbepoetin (novel erythropoiesis stimulating protein (NESP)). This is the basis of the method currently used to detect misuse of rEPO and NESP by elite athletes. Recently, an unknown activity has been attributed to some urine samples (denominated 'unstable' urine by the ...