Na+,K+-ATPase activity, phosphorylation, and [3H]ouabain binding in sarcolemma isolated from spontaneously hypertensive rat (SHR) hearts were compared to the same parameters in sarcolemma from normotensive rat (WKY) hearts. Sarcolemma prepared from SHR heart contained significantly less ouabain-inhibitable ATPase activity than sarcolemma from WKY heart. No significant differences in sarcolemmal...

Well-known aminoacyl-tRNA synthetase (ARSase) inhibitors, namely the analogues of amino acids and aminoacyl adenylates (aminoalkyl- and aminophosphonyl adenylates with Ki congruent to 0.1 microM) as well as the diadenosine 5',5'''-p1,p4-tetraphosphate (Ap4A) phosphonoanalogues, were for the first time used for the Ap4A biosynthesis regulation. Effects of a set of such compounds on lysyl-, pheny...

Albizia richardiana, a fast-growing, large deciduous tree belonging to the Fabaceae family, grows well in hot and humid areas but mainly tropics of Old World. The medicinal other uses richardiana are documented, phytotoxic effects this have not yet been investigated. We conducted study investigate activity leaves identify growth inhibitory substances for controlling weeds sustainable way. Aqueo...

Traditional medicines have been used in both developed and developing countries for a long time, one of which is longevity spinach (Gynura procumbens). Its plants are type plant that by the community to treat various diseases with their flavonoid content. This produces flavonoids, such as quercetin, can be optimized providing shade increasing nitrogen nutrients giving biochar fertilizing. study...

The peroxidase activity of prostaglandin H (PGH) synthase catalyzes reduction of 5-phenyl-4-pentenyl hydroperoxide to 5-phenyl-4-pentenyl alcohol with a turnover number of approximately 8000 mol of 5-phenyl-4-pentenyl hydroperoxide/mol of enzyme/min. The kinetics and products of reaction establish PGH synthase as a classical heme peroxidase with catalytic efficiency similar to horseradish perox...

During arousal from estivation in land snails, Otala lactea, active metabolic functions are restored within minutes and oxygen consumption increases dramatically. During the transition from the hypoxic conditions of estivation to normoxia it is possible that xanthine oxidase (XO) in hepatopancreas contributes to the observed lipid peroxidation. Using a fluorometric assay that is based on the ox...

The phytotoxic potential of plants and the effect their active components on another plant species is being explored as a alternative to synthetic herbicides for weed control. In current study, we investigated leaves Dregea volubilis (L.f.) Benth. ex Hook.f. against four test [timothy (Phleum pratense L.), barnyard grass (Echinochloa crus-galli (L.) P. Beauv), lettuce (Lactuca sativa alfalfa (M...

The allelopathic potential of plant species and their related compounds has been increasingly reported to be biological tools for weed control. Garcinia xanthochymus was assessed against several test species: lettuce, rapeseed, Italian ryegrass, timothy. extracts G. leaves significantly inhibited all the plants in a concentration- species-specific manner. Therefore, identify specific involved a...

The release of glucokinase from digitonin-permeabilized hepatocytes shows different characteristics with respect to ionic strength and [MgCl2] from the release of other cytoplasmic enzymes. Release of glucokinase is most rapid at low ionic strength (300 mM sucrose, 3 mM Hepes) and is inhibited by increasing concentration of KCl [concn. giving half-maximal inhibition (I50) 25 mM] or Mg2+ (I50 0....

Juvenile hormone esterase (JHE) from the serum of the cricket, Gryllus assimilis, was purifi ed to homogeneity in a four-step procedure involving polyethylene glycol precipitation, hydrophobic interaction FPLC, and ion exchange FPLC. This procedure could be completed in 4 days and resulted in a greater than 900-fold purifi cation with greater than 30% recovery. The purifi ed enzyme exhibited a ...