Methyl-CpG binding domain (MBD) proteins have been shown to couple DNA methylation to transcriptional repression. This biological property suggests a role for MBD proteins in the silencing of tumor suppressor genes that are hypermethylated at their promoter CpG islands in cancer cells. Despite the demonstration of the presence of MBDs in the methylated promoter of several genes, we still ignore...

Objective. To identify DNA methylation patterns of heavy smokers in oral rinse samples. Methods. Genome-wide data was imported from Gene Expression Omnibus GSE70977 using the GEOquery package. Two independent sets were analyzed: (a) 71 epigenomes cancer-free subjects (heavy n = 37 vs. non-smokers 31); for concordance assessment (b) 139 oral-cancer patients’ 92 47). Differential CpG positions an...

Abnormal hypermethylation of DNA is highly detected in many cancer types. It is accepted that the hypermethylation contributes to tumor developing by silencing the tumor suppressor genes expression. New data techniques, Illumina Infinium HumanMethylation450, gives us a wide approach about genome methylation density and location. 8 different TCGA cancer data were compared. As a result, hypomethy...

Abstract BACKGROUND Glioblastoma patients showing hypermethylation of the promoter O6-methylguanine-methyltransferase (MGMT) gene have significantly improved survival when treated with temozolomide compared to hypomethylated MGMT promoters. However, prognostic and predictive significance partial methylation is unclear. METHODS The National Cancer Database was queried for newly diagnosed in 2018...

CpG island promoter hypermethylation of tumor suppressor genes is a common hallmark of human cancer, and new large-scale epigenomic technologies might be useful in our attempts to define the complete DNA hypermethylome of tumor cells. Here, we report a functional search for hypermethylated CpG islands using the colorectal cancer cell line HCT-116, in which two major DNA methyltransferases, DNMT...

Histone H3 lysine 9 (H3K9) and lysine 27 (H3K27) trimethylation are properties of stably silenced heterochromatin whereas H3K9 dimethylation (H3K9me2) is important for euchromatic gene repression. In colorectal cancer cells, all of these marks, as well as the key enzymes which establish them, surround the hMLH1 promoter when it is DNA hypermethylated and aberrantly silenced, but are absent when...

BACKGROUND The genetic pathways of aggressive changes of bone tumors are still poorly understood. It is very important to analyze DNA copy number alterations (DCNAs), to identify the molecular events in the step of progression to the aggressive change of bone tissue. METHODS Genome-wide array-based comparative genomic hybridization (array CGH) was used to investigate DCNAs of 14 samples from ...

BACKGROUND MicroRNAs are associated with tumor genesis and progression in various carcinomas. MicroRNA-148a (miR-148a) was reported to have low expression in gastrointestinal cancers, and might be regulated by promoter region DNA methylation. METHODS Bisulfite-modified sequencing was used to determine the promoter region DNA methylation status of human gastrointestinal cancer cell lines. Expr...

background :many breast cancer tumor suppressor genes have been reported to undergo hypermethylation, including er, rassf1a, hic, cdh1, gspt1, apc, twist and ccnd2. we first determined the promoter methylation of the above eight tumor suppressor genes as epigenetic markers of breast cancer in three cell lines with different er status. methods : this study was an experimental study performed on ...