Investigations have been made to develop an efficient protocol for identification and quantification of phenolic acids and flavonoids in hairy roots (HR) of Hypericum perforatum L. HR were induced from root segments of in vitro grown seedlings from H. perforatum, after co-cultivation with Agrobacterium rhizogenes A4. Transgenic status of HR was confirmed by PCR analysis using rolB specific prim...

The essential oil isolated from fresh aerial parts of Hypericum perforatum L. was analyzed by GC and GC/MS. One hundred and thirty four identified compounds accounted for 98.7% of the total oil. The main components of the oil were: germacrene D (18.6%), (E)-caryophyllene (11.2%), 2-methyloctane (9.5%), α-pinene (6.5%), bicyclogermacrene (5.0%) and (E)-β-ocimene (4.6%). The volatile profile of H...

BACKGROUND A number of bio-active secondary metabolites have been identified and reported for several Hypericum species. Many studies have reported the potential use of the plant extracts against several pathogens. However, Hypericum triquetrifolium is one of the least studied species for its antimicrobial activity. The aim of the present study was to evaluate the cytotoxic effect of the essent...

BACKGROUND St John's wort (Hypericum perforatum) has been known for centuries for its therapeutic properties and its efficacy as an antidepressant has been confirmed by a growing body of evidence. During the last two decades it has also come to prominence with a wider public, due to advertising efforts across Europe and United States of America. However, its availability without prescription, a...

Hypericum perforatum, is a plant which blooms between july and september at farms, borders of roads and woods, top of hills and grasslands, whose anti-inflammatory effects are shown in various studies. It is shown that cigarette smoke causes increase in inflammation mediators on lung. Due to this reason, we planned a study to examine the protective effects of Hypericum perforatum aganist smoke ...

Several modes of reproduction were described in the genus Hypericum. Besides obligate sexuality, irregularities in meiosis, polyembryony and pseudogamous apomixis were noticed in various taxa of the genus. This review summarizes previous knowledge and also analyses evolutionary consequences of different breeding systems and possible employment of particular reproductive modes for breeding in th...

Stamenković, J., Radojković, I., Đorđević, A., Jovanović, O., Petrović, G., Stojanović, G.: Optimization of HPLC method for the isolation of Hypericum perforatum L. methanol extract. Biologica Nyssana, 4 (12), December 2013: 81-85. St. John's Wort (Hypericum perforatum L.) is one of the most studied plant species in the family Hypericaceae. The aim of this study was the identification of the co...