The observation that hydroxylamine inhibits xanthine oxidase activity through interference with the dehydrogenase portion of the enzyme (1) led us to determine whether hydroxylamine forms a chemical complex with riboflavin or its biological derivatives, riboflavin-5phosphate (FMN) and flavin adenine dinucleotide (FAD). These studies were stimulated by those of Kaplan and his group, who explaine...

The possibility of hydroxylamine electrochemical determination over a CoO(OH)-modified electrode, accompanied by complex-formation, has been studied from the theoretical point of view. The correspondent mathematical model has been developed and analyzed by means of linear stability theory and bifurcation analysis. It was shown that CoO(OH) may be applied as an efficient electrode modifier i...

In the present paper, the electrochemical properties of magnetic iron oxide nanoparticles (Fe2O3 and Fe3O4) as highly sensitive sensors for the simultaneous determination of hydrazine and hydroxylamine was described. The electrochemical behavior of hydrazine and hydroxylamine was investigated using cyclic voltammetry, chronoamperometry and differentia...

Hydroxylamine has been determined by its oxidation to nitrite with a known excess of bromine. Bromine in acidic medium bleaches the dye methyl red. A known excess of bromine when treated with hydroxylamine is reduced to bromide and the unreacted bromine is determined using methyl red. The method obeys Beer’s law in the range 0-5 μg of hydroxylamine in an overall aqueous volume of 25 mL. The rel...

(+)- And (-)-amphetamine and methamphetamine were N-oxygenated by the cDNA expressed adult human flavin-containing monooxygenase form 3 (FMO3), their corresponding hydroxylamines. Two major polymorphic forms of human FMO3 were studied, and the results suggested preferential N-oxygenation by only one of the two enzymes. Chemically synthesized (+/-)-amphetamine hydroxylamine was also a substrate ...

Hydrogen sulfide (H2S) plays a crucial role in the regulation of blood pressure and oxidative stress. In the present study, we tested the hypothesis that H2S exerts its cardiovascular effects by reducing oxidative stress via inhibition of NADPH oxidase activity in the rostral ventrolateral medulla (RVLM). We examined cell distributions of cystathionine-β-synthase (CBS) and effects of H2S on rea...

[adenine-U-14C]ADP-ribose-agmatine and [adenineU-’4C]ADP-ribose-histone were synthesized by an NAD:arginine ADP-ribosyltransferase from [’*C]NAD and agmatine and histone, respectively. The pseudofirst order rate constants for breakdown of the two components either in 0.4 N NaOH or in 0.4 M neutral hydroxylamine were identical. Hydroxylamine treatment of [‘4C]ADP-ribose-agmatine or [32P]ADP-ribo...

Two different silver colloids were prepared by chemical reduction of silver nitrate with trisodium citrate and hydroxylamine hydrochloride to compare their characteristics in relation to their possible use in surface-enhanced Raman scattering (SERS) spectroscopy. The morphology and plasmon resonance of the single nanoparticles and aggregates integrating these colloids were characterized by mean...

The intermediate role of hydroxylamine in nitrate assimilation has been suggested by observations that different organisms form ammonia from hydroxylamine in vivo, and that hydroxylamine occurs in nutrient media and cells. In further support are recent investigations describing enzymatic preparations which catalyze the reduction of hydroxylamine to ammonia. Taniguchi et al. (J. Biochem. (Japan)...