For discovering the components that contributed to the bonding strength of the glue substances produced by appressoria of Pyricularia oryzae on wax-coated cover-glasses, the influences of metabolic inhibitors and hydrolytic enzymes were investigated. The bonding strength of appressoria was assessed by the ratio of the remaining appressoria after sonication to the appressoria formed before sonic...

BACKGROUND The production of bioethanol from lignocellulosic feedstocks is dependent on lignocellulosic biomass degradation by hydrolytic enzymes. The main component of lignocellulose is cellulose and different types of organisms are able to secrete cellulases. The filamentous fungus Aspergillus nidulans serves as a model organism to study cellulase production and the available tools allow expl...

The prokaryotic residents of the Tis solar saltern in the southeast of Iran on the shore of Oman Sea were investigated by the culture-dependent methods. Sequencing of the PCR-amplified fragments of 16S rRNA genes revealed that bacterial populations were related to Actinobacteria, Bacteroidetes, Balneolaeota, Firmicutes, and Proteobacteria. T...

The recent discovery of IsPETase, a hydrolytic enzyme that can deconstruct poly(ethylene terephthalate) (PET), has sparked great interest in biocatalytic approaches to recycle plastics. Realization commercial use will require the development robust engineered enzymes meet demands industrial processes. Although rationally PETases have been described, experimentally optimized via directed evoluti...

Halophilic bacteria are a group of microorganisms living in saline environments and some cases need the salinity to survive. Furthermore,these species have potential for interesting promising applications. In fact, this is due not only their ability adapt extreme physico-chemical conditions but also because many them they produce substances industry such as extracellular hydrolytic enzymes.The ...

Fifteen bacterial monocultures were isolated from lubricant-contaminated wastewater of an electric power station in Sofia. Six isolates showed best growth in liquid media with 1.5% hexadecane, and on mineral salt agar plates supplemented with one of the following hydrocarbons: n-hexadecane, paraffin, kerosene and samples of wastewater. The ability of all isolates to produce extracellular hydrol...

BACKGROUND Mucinases and sialidases contribute to the process of invasion and colonisation in many conditions and infections of the female reproductive tract by degrading the protective cervical mucus. The role of hydrolytic enzymes in the pathogenesis of sexually transmitted diseases and their effect on cervical mucus are discussed in this review. METHODS Articles were searched for using the...

The sweat eccrine and apocrine glands of human skin secrete many functionally significant substances, including hydrolytic enzymes, onto its surface through the mechanisms secretion, excretion recruitment. Shown is their recreational origin as a secretory product digestive glands—pepsinogen by stomach glands, α-amylase—by pancreas. Hydrolases in epidermal barrier provide hydrolysis proteins, ca...

Candida albicans is a fungus that often colonizes the human body as commensal without causing any symptoms. Several C. virulence factors have been described in literature, of which best known are adhesion to host mucosa cells and secretion hydrolytic enzymes. The transition from colonization infection depends not only on fungus, but also response invasion. This literature review focuses differe...

Midgut hydrolytic enzymes of Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) were identified and partially characterized. Km, Vmax, optimum pH, and specific activity were determined for proteolytic enzymes and αamylases. All hydrolytic enzyme activity had an optimum pH value in the alkaline pH range. We observed major serine protease activity, together with minor cysteine-like activity, th...