Leptospirosis is a zoonosis of worldwide distribution, caused by Leptospira interrogans and is considered as an emerging global public health problem. Transmission usually results from direct or indirect exposure to the urine or other body fluids of leptospiruric animals which may become a source of infection for human or other animals. Having a humid climate with plenty of annual rainfall, Gui...

BACKGROUND Human schistosomiasis remains a serious worldwide public health problem. At present, a sensitive and specific assay for routine diagnosis of schistosome infection is not yet available. The potential for detecting schistosome-derived DNA by PCR-based methods in human clinical samples is currently being investigated as a diagnostic tool with potential application in routine schistosomi...

Human urine contains growth factors; their physiological roles have not been established. The effect of normal human urine was examined in vitro on clonal growth of human bladder cancer cell lines. Clonal growth of HT-1376, HT-1197, and T24 was enhanced by five different fresh human urine samples from young men. Colony stimulating activity was detected in fractions with a molecular weight great...

background: childhood masturbation (cm) is considered a variant of normal sexual behavior; however, it is commonly misdiagnosed as epilepsy and movement disorders. as the first study from iran, we analyzed a large population of infants and children with cm in a case-control study and evaluated the value of mucus in urine analysis as an alternative diagnostic tool for cm. methods: a total of 623...

Concurrent methods for identification of urine as being of human origin, and for DNA-typing from small stains of human urine were examined. A urine stain was extracted with phosphate-buffered saline (PBS), and the extract was filtered using a Centricon-100 device. The filtrate was subjected to electrospray ionization liquid chromatography-mass spectrometry (ESI-LC-MS) for identification of huma...

An internally standardized method for the determination of 3-methylhistidine in human urine is presented. This methylated amino acid and the chemically analogous internal standard 3-ethylhistidine were isolated from human urine specimens using small columns of cation-exchange resin. Quantification was accomplished by high-performance liquid chromatography using post-column derivatization with o...

myiasis is the infestation of organs and tissues of human and animals with fly larvae. this article reports an 18 year-old man with urogenital myiasis, the passing of live megaselia scalaris larvae in the urine, from zanjan city, northwest of iran. we discourse the importance of diagnosis and management of urogenital myiasis in medicine.

The stability of rat (human) CRF in serum, urine and tissue incubation medium was examined using Sephadex gel filtration and CRF radioimmunoassay with anti-rat (human) CRF serum. Human serum after incubation with rat (human) CRF for 1 h at 37 degrees C showed two peaks of CRF immunoreactivity on a Sephadex G-50 fine column. Most of the immunoreactivity coeluted with the rat (human) CRF marker. ...

An analytical method for analyzing SMZ in human plasma and urine using high-performance liquid chromatography (HPLC) was developed. The separation carried out on a Shim-pack GIST® C18 column (150 × 4.6 mm, 5 ?m) with temperature setting of 30°C. mobile phase consisted glacial acetic acid pH 2.5: methanol: acetonitrile (70:25:5, v/v/v) at rate 0.8 mL/min. Detection done PDA detector the range ?1...