نتایج جستجو برای: human sperm

تعداد نتایج: 1672807  

2014
David S Karabinus Donald P Marazzo Harvey J Stern Daniel A Potter Chrispo I Opanga Marisa L Cole Lawrence A Johnson Joseph D Schulman

BACKGROUND Flow cytometric sorting can be used to separate sperm based on sex chromosome content. Differential fluorescence emitted by stained X- vs. Y-chromosome-bearing sperm enables sorting and collection of samples enriched in either X- or Y-bearing sperm for use to influence the likelihood that the offspring will be a particular sex. Herein we report the effectiveness of flow cytometric so...

2017
Violeta Calle-Guisado Ana Hurtado de Llera David Martin-Hidalgo Jose Mijares Maria C Gil Ignacio S Alvarez Maria J Bragado Luis J Garcia-Marin

AMP-activated kinase (AMPK), a protein that regulates energy balance and metabolism, has recently been identified in boar spermatozoa where regulates key functional sperm processes essential for fertilization. This work's aims are AMPK identification, intracellular localization, and their role in human spermatozoa function. Semen was obtained from healthy human donors. Sperm AMPK and phospho-Th...

Journal: :Journal of proteomics 2013
Gaigai Wang Yueshuai Guo Tao Zhou Xiaodan Shi Jun Yu Ye Yang Yibo Wu Jing Wang Mingxi Liu Xin Chen Wenjiao Tu Yan Zeng Min Jiang Suying Li Pan Zhang Quan Zhou Bo Zheng Chunmei Yu Zuomin Zhou Xuejiang Guo Jiahao Sha

The male gamete (sperm) can fertilize an egg, and pass the male genetic information to the offspring. It has long been thought that sperm had a simple protein composition. Efforts have been made to identify the sperm proteome in different species, and only about 1000 proteins were reported. However, with advanced mass spectrometry and an optimized proteomics platform, we successfully identified...

2014
Christoph Brenker Yu Zhou Astrid Müller Fabio Andres Echeverry Christian Trötschel Ansgar Poetsch Xiao-Ming Xia Wolfgang Bönigk Christopher J Lingle U Benjamin Kaupp Timo Strünker

Sperm are equipped with a unique set of ion channels that orchestrate fertilization. In mouse sperm, the principal K(+) current (IKSper) is carried by the Slo3 channel, which sets the membrane potential (Vm) in a strongly pHi-dependent manner. Here, we show that IKSper in human sperm is activated weakly by pHi and more strongly by Ca(2+). Correspondingly, Vm is strongly regulated by Ca(2+) and ...

2015
Yoku Kato Yoshikazu Nagao Xiuchun (Cindy) Tian

Intracytoplasmic sperm injection (ICSI) has become the method of choice to treat human male infertility. One of the outstanding problems associated with this technique is our current lack of knowledge concerning the effect of sperm capacitation and motility upon the subsequent development of oocytes following ICSI. In the present study, we first examined the capacitation state of sperm exhibiti...

2011
Ana Paula Sousa Alexandra Amaral Marta Baptista Renata Tavares Pedro Caballero Campo Pedro Caballero Peregrín Albertina Freitas Artur Paiva Teresa Almeida-Santos João Ramalho-Santos

Human sperm samples are very heterogeneous and include a low amount of truly functional gametes. Distinct strategies have been developed to characterize and isolate this specific subpopulation. In this study we have used fluorescence microscopy and fluorescence-activated cell sorting to determine if mitochondrial function, as assessed using mitochondrial-sensitive probes, could be employed as a...

2014
Kun Li Yamei Xue Aijun Chen Youfang Jiang Haifeng Xie Qixian Shi Songying Zhang Ya Ni

Heat shock protein 90 plays critical roles in client protein maturation, signal transduction, protein folding and degradation, and morphological evolution; however, its function in human sperm is not fully understood. Therefore, our objective in this study was to elucidate the mechanism by which heat shock protein 90 exerts its effects on human sperm function. By performing indirect immunofluor...

2015
Hasmik Margaryan Andriy Dorosh Jana Capkova Pavla Manaskova-Postlerova Anatoly Philimonenko Pavel Hozak Jana Peknicova

BACKGROUND Sperm proteins are important for the sperm cell function in fertilization. Some of them are involved in the binding of sperm to the egg. We characterized the acrosomal sperm protein detected by a monoclonal antibody (MoAb) (Hs-8) that was prepared in our laboratory by immunization of BALB/c mice with human ejaculated sperms and we tested the possible role of this protein in the bindi...

Journal: :Journal of cell science 2007
Philip C N Chiu Man-Kin Chung Riitta Koistinen Hannu Koistinen Markku Seppala Pak-Chung Ho Ernest H Y Ng Kai-Fai Lee William S B Yeung

Fertilization depends on successful binding of the spermatozoa to the zona pellucida of the oocyte. Glycodelin-A inhibits spermatozoa-zona pellucida binding. Previous data showed that glycodelin-A receptor(s) and zona pellucida protein receptor(s) on human spermatozoa are closely related. Using a chemical cross-linking approach, the glycodelin-A-sperm receptor complex was isolated. The receptor...

2015
Analilia Saldívar-Hernández María E. González-González Ana Sánchez-Tusié Israel Maldonado-Rosas Pablo López Claudia L. Treviño Fernando Larrea Mayel Chirinos

BACKGROUND The mammalian oocyte extracellular matrix known as the zona pellucida (ZP) acts as a barrier to accomplish sperm fusion with the female gamete. Although penetration of the ZP is a limiting event to achieve fertilization, this is one of the least comprehended stages of gamete interaction. Even though previous studies suggest that proteases of sperm origin contribute to facilitate the ...

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