Many young cancer patients are now being given the option to store ovarian cortical biopsies before undergoing potentially damaging chemo- or radiotherapy. This tissue mainly contains large numbers of immature primordial follicles. Currently the only option to restore fertility using this tissue is by transplantation which may not be a viable option for all patients. Greater options to realise ...

background: testis tissue xenografting and the resultant sperm in a xenograft may provide a unique approach to rescue the genetic material of males that die prematurely and is a model for the study of human spermatogenesis and can represent an alternative approach for fertility preservation in cancer patients. this study was aimed to evaluate the xenogenic dog sperm in formation of male pronucl...

The CD44 family belongs to a larger group of hyaluronic acid-binding proteins and plays important roles in oocyte maturation, fertilization and preimplantational embryo development. We analyzed the CD44 receptor in sheep oocytes and embryos. Immature oocytes (N = 66) were obtained from a local abattoir; mature oocytes (N = 35) and embryos (N = 41) were obtained by laparotomy from adult hai...

In vitro fertilization, one of assisted reproduction`s technique is, for some couples, the only possibility to conceive a child. One of the main condition that must be fulfield in order to reach this goal is the quality of human oocytes. The ovarian stimulation protocols used have as an important goal the oocytes quality. The maturity grade of the oocytes is a good indicator of their quality.Th...

Background Although ovarian tissue cryopreservation and transplantation is used for fertility preservation in patient undergoing cytotoxic treatments but obtaining good quality oocyte is still a debate. Xenotransplantation has been introduced as a reliable technique for better understanding of the transplantation conditions, but to achieve healthy embryos, the quality of obtained oocytes need t...

ECAT1 is a subunit of the subcortical maternal complex that is required for cell cycle progression during pre-implantation embryonic development; however, its exact function remains to be elucidated. Here we investigated the expression of ECAT1 in human ovarian tissue, oocytes and pre-implantation embryos and assessed its function by using RNA interference (RNAi) in oocytes. ECAT1 mRNA was high...

OBJECTIVE In order to increase the number of mature oocytes usable for intracytoplasmic sperm injection (ICSI), we aimed to investigate the effect of co-culturing granulosa cells (GCs) on human oocyte maturation in vitro, the fertilization rate, and embryo development. METHODS A total of 133 immature oocytes were retrieved and were randomly divided into two groups; oocytes that were cultured ...

OBJECTIVE To study the intrinsic fertility of the human oocyte. DESIGN A large retrospective study of natural cycle single embryo transfer (ET) IVF cycles. SETTING Private IVF clinic, university, and private hospital. PATIENT(S) Patients were enrolled consecutively over an 8-year period in a single ET natural cycle protocol. INTERVENTION(S) A total of 13,949 oocyte retrievals with natur...

Sixty unfertilized human oocytes and two fresh polar bodies were karyotyped by spectral karyotyping (SKY). The oocytes were provided by 29 women ranging from 30 to 42 yr of age. The mean hybridization efficiency for oocytes was 95.2% (60/63). Nondisjunction of bivalent chromosomes (13.3%) and predivision of sister chromatids at meiosis I (3.3%) were unequivocally determined by analysis first wi...

BACKGROUND The development competence of human oocytes declines with increasing age. The objective of this study was to investigate the effect of age on gene expression profile in mature human oocytes. METHODS mRNA was isolated for whole genome gene expression microarray analysis from metaphase II (MII) oocytes donated by IVF or ICSI patients [10 women aged <36 years (younger) and five women ...