Human oncolytic adenoviruses have been used in clinical trials targeting cancers of epithelial origin. To gain a better understanding of the infectious cycle of adenovirus in normal human squamous tissues, we examined the viral infection process in organotypic cultures of primary human keratinocytes. We show that for the infection to occur, wounding of the epithelium is required. In addition, i...

Different methods of molecular hybridization were used to study DNA sequences of the highly oncogenic simian adenovirus SA7 (C8) present in the genomes of two transformed rat cell lines and in cells from three hamster tumours induced by adenovirus SA7. The entire DNA or the left-hand terminal SalI C fragment (19.5% of the genome) were employed. All cell lines retained an intact left-hand region...

The biological properties of a human adenovims that has incorporated defective SV40 genetic material within its adenocapsid have been extensively studied (1, 2). The particle containing the defective SV40 genome is known as PARA (2). Its presence confers new properties on the adenovirus population which now replicates in simian cells (3), carries the genetic information for the synthesis of SV4...

Humanized mice have emerged as a promising model to study human immunity in vivo. Although they are susceptible to many pathogens exhibiting an almost exclusive human tropism, human immune responses to infection remain functionally impaired. It has recently been demonstrated that the expression of HLA molecules improves human immunity to lymphotropic virus infections in humanized mice. However,...

Human adenoviruses are large (150 MDa) nonenveloped double-stranded DNA (dsDNA) viruses that cause acute respiratory, gastrointestinal and ocular infections. Despite these disease associations, adenovirus has aided basic and clinical research efforts through studies of its association with cells and as a target of host antiviral responses. This review highlights the knowledge of adenovirus disa...

UNLABELLED Adenovirus vectors are widely used as vaccine candidates for a variety of pathogens, including HIV-1. To date, human and chimpanzee adenoviruses have been explored in detail as vaccine vectors. The phylogeny of human and chimpanzee adenoviruses is overlapping, and preexisting humoral and cellular immunity to both are exhibited in human populations worldwide. More distantly related ad...

introduction: hela cell is a widely studied cell line derived from human cerzix adenocarcinoma. the cells are epithelial - like in morphology and susceptible to polio viruses and adenovirus type 5. hela cells are usually as monolayer for isolation and propagation of some human viruses, as well as for the expression of recombinant proteins, including hepatitis b surface antigen. the aim of this ...

Human adenovirus has evolved to infect and replicate in terminally differentiated human epithelial cells, predominantly those within the airway, the gut, or the eye. To overcome the block to viral DNA replication present in these cells, the virus expresses the Early 1A proteins (E1A). These immediate early proteins drive cells into S-phase and induce expression of all other viral early genes. D...

UNLABELLED For DNA viruses, genetic recombination, addition, and deletion represent important evolutionary mechanisms. Since these genetic alterations can lead to new, possibly severe pathogens, we applied a systems biology approach to study the pathogenicity of a novel human adenovirus with a naturally occurring deletion of the canonical penton base Arg-Gly-Asp (RGD) loop, thought to be critic...

Joanne M. Wroblewski lez-Garcia M, Koedens M, Fukunga N, Davidson B, Dick J, Minn A, Boise L, Thompson C, Wicha M, Nunez G: A recombinant bclL. Travis Lay xs adenovirus selectively induces apoptosis in cancer cells but not Timothy C. Meeker in normal bone marrow cells. Proc Natl Acad Sci USA 92:11024, Markey Cancer Center 1995 University of Kentucky 4. Wroblewski J, Lay L, Van Zant G, Phillips ...