نتایج جستجو برای: hrp immunoassay
تعداد نتایج: 20751 فیلتر نتایج به سال:
The endocytosis of ricin, horseradish peroxidase (HRP), and a conjugate of ricin-HRP by monolayer cultures of murine neuroblastoma was studied using morphological and biochemical techniques. The binding of (125)I-ricin and (125)I-ricin-HRP to cells at 4 degrees C, as a function of ligand concentration, was a saturable process. The apparent affinity constants, determined at equilibrium, were 2.8...
COVID-19 has evolved into a global pandemic. Early and rapid detection is crucial to control of the SARS-CoV-2 transmission. While representing gold standard for early diagnosis, nucleic acid tests are often complicated time-consuming. Serological antibody characterized by high rates false-negative diagnoses, especially during infection. Here, we developed novel nanozyme-based chemiluminescence...
To examine the involvement of (pro)renin receptor in the accelerated organ damage in streptozotocin-induced diabetic male SHRsp, the rats fed a high-salt diet were divided into 5 groups: a group treated with the vehicle, a group treated with 15 mg/kg/day of imidapril (ACEi), a group treated with 60 mg/kg/day of imidapril (High ACEi), a group treated with handle region peptide (HRP), and a group...
The C1a isoenzyme of horseradish peroxidase (HRP) is an industrially important heme-containing enzyme that utilizes hydrogen peroxide to oxidize a wide variety of inorganic and organic compounds for practical applications, including synthesis of fine chemicals, medical diagnostics, and bioremediation. To develop a ultra-high-throughput screening system for HRP, we successfully produced active H...
To accurately diagnose COVID-19 infection and its time-dependent progression, the rapid, sensitive, noninvasive determination of immunoglobulins A specific to SARS-CoV-2 (IgA) in saliva serum is needed complement tests that detect G M. We have developed a dual optical/chemiluminescence format lateral flow immunoassay (LFIA) immunosensor for IgA saliva. recombinant nucleocapsid antigen specifica...
The extent of exocytosis of pinocytic vesicle contents was studied in suspension-cultured Chinese hamster ovary (CHO) cells using horseradish peroxidase (HRP) as a pinocytic content marker. HRP was shown to be internalized via fluid-phase pinocytosis in CHO cells. After an HRP pulse of 2.5-10 min a rapid decrease of 30-50% in cell-associated HRP activity was observed within 10-20 min at 37 degr...
Surface immunoglobulin (Ig)-mediated endocytosis has been investigated in rat B lymphocytes and plasma cells, using horseradish peroxidase (HRP)-labeled sheep anti-rat Ig Fab' fragment of antibody and HRP as monomeric ligands, respectively. Quantitative estimates of HRP activity associated either with plasma membrane or with endomembrane compartments were made in several experimental conditions...
We report on the surprising loss of transganglionic and retrograde labeling in the spinal cord of the rat after co-injection of the tracers wheat germ agglutinin-HRP (WGA-HRP) and choleragenoid toxin-HRP (CTB-HRP) into the sciatic nerve. Injection of WGA-HRP alone produced a pattern of transganglionic label consistent with transport by small-diameter primary afferent fibers. Small cell bodies w...
Improving human resources performance (HRP) is the most important task of the century. However, we have few measures or management interventions to make such improvement possible; although we cannot identify the pattern to be followed by human resources because systems and processes in an organization are often regarded as a kiss of death to encouraging creativity. For improving human resource ...
Amperometric hydrogen peroxide biosensors were fabricated by incorporating horseradish peroxidase (HRP) into poly[pyrrole-co-[4-(3-pyrrolyl)butanesulfonate]] (Py-PS) copolymer films deposited on an SnO2 electrode surface by electropolymerization. The HRP/Py-PS electrodes exhibited an extended dynamic range and a markedly improved operational and storage stability, compared with HRP-incorporated...
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