High-performance liquid chromatography (HPLC) methods employing ultraviolet (UV) detector are not sufficiently sensitive to measure the low plasma concentrations following single oral dose of imipramine. Therefore, in the present study a simple, rapid and yet sensitive HPLC method with UV detection was developed and validated for quantitation of imipramine in human plasma samples. An efficient ...

Caffeine intake by pregnant women, adults and children can be harmful to the health of all particularly fetuses if exceeds permissible limits. Therefore, it is fundamental importance measure its concentration accurately using certified reference materials (CRMs). In literature, no scientific details are published about certification caffeine standard solutions, therefore, present article covers...

In the pharmaceutical industry, an important step consists in the removal of possible drug residues from the involved equipments and areas. The cleaning procedures must be validated and methods to determine trace amounts of drugs have, therefore, to be considered with special attention. An HPLC–UV method for the determination of digoxin residues on stainless steel surfaces was developed and val...

The aim of this study was to compare the significance of the intestinal hydrolysis of prenylated flavonoids in Herba Epimedii by an intestinal enzyme and flora. Flavonoids were incubated at 37 °C with rat intestinal enzyme and intestinal flora. HPLC-UV was used to calculate the metabolic rates of the parent drug in the incubation and LC/MS/MS was used to determine the chemical structures of met...

BACKGROUND AND AIMS Bilirubin is an orange-yellow tetrapyrrole produced from the breakdown of heme by mammals and some other vertebrates. Plants, algae and cyanobacteria synthesize molecules similar to bilirubin, including the protein-bound bilins and phytochromobilin which harvest or sense light. Recently, we discovered bilirubin in the arils of Strelitzia nicolai, the White Bird of Paradise T...

Hydrolysis of whey protein concentrates (WPCs) at different temperatures and pHs, using three enzymes: pepsin, trypsin, and Alcalase, was monitored during more than 5 hr by reversed phase HPLC/UV,

BACKGROUND Tramadol ((±)-TRA) is recommended for the treatment of mild to moderate cancer pain by the World Health Organization. An oral liquid formulation of (±)-TRA is preferable for patients with a compromised swallowing function. However, the stability of (±)-TRA in aqueous solution has yet to be determined in a clinical setting. The aim of this study was to evaluate the photostability of (...

Two cultivars of fresh market tomato [Oregon Spring (OS) and Red Sun (RS)] were each grown in two high tunnels at Beltsville, MD covered with a contrasting material of similar thickness (0.152mm) and durability (4-year polyethylene). One covering material (Tyco Tufflite IV) transmitted ambient solar UV radiation from 290 to 400nm (designated+UV) while the other material (Dura-Film Super 4) bloc...

A novel approach to identify structures and quantify the absolute amounts of individual ligands on multi-functionalized gold nanoparticles by an FTIR and HPLC/MS/UV/CLND method has been developed.

Allixin, a phytoalexin isolated from garlic, was induced by irradiating fresh garlic cloves with sunlight or UV light. Induced allixin was analyzed by HPLC, and the accumulated amounts of allixin were 3.1-6.3 microg/g under experimental conditions.