We previously established an analysis method for (measuring) determining the cholesterol levels of 5 major lipoprotein classes (HDL, LDL, IDL, VLDL and chylomicrons) in serum by an anion-exchange high-performance liquid chromatography (AEX-HPLC) method, but lipoprotein(a) [Lp(a)], a well-known risk factor for atherosclerotic diseases, was not determinable. Therefore, we have established new AEX...

Antennaria dioica ( Asteraceae family) – is a perennial herb, commonly found in dry grasslands and sandy or stony places from Eurasian areas. It known traditional medicine as antioxidant, diuretic, choleretic anti-inflammatory remedy. This species should be reconsidered possible sources of phenols, mainly flavonoids hydroxycinnamic acids. Thus, the aim this study was to validate chromatographic...

BACKGROUND Monitoring whole-blood concentrations of cyclosporin A (CsA) is common practice in the management of solid organ and bone marrow transplant recipients. In a multicenter study we evaluated a new, direct (no pretreatment) CsA assay on the Dade Behring Dimension RxL system and compared results with those from the Abbott TDx CsA immunoassay and a HPLC method. METHODS Whole-blood sample...

Abstract Background In humans, various diseases are associated with the accumulation of free radicals. The antioxidants can scavenge radicals and reduce their impact; thus, search for effective natural plant origin is indispensable. present study aims to determine, first time, flavonoid compounds profile investigate radical scavenging antioxidant properties methanolic extract Taxus baccata L. f...

We evaluated three newly introduced systems for automated determinations of hemoglobin (Hb) A1c, which allow the processing of large amounts of samples in a routine clinical laboratory. We compared these methods--the Variant HPLC, the Hi-Auto A1c analyzer system, and the Roche immunoassay--with the Diamat HPLC system. All showed good precision and good concordance with the Diamat HPLC. The refe...

The present study reports the development and validation of a microbiological assay. To assess this methodology, method was developed validated for quantification CEF by high performance liquid chromatography (HPLC). microbial assay diffusion in 3x3 cylinder agar presented showed satisfactory results as to specificity, linearity range 2.0 - 8.0 ?g.mL-1, precision (109.42 %), accuracy (102.3 rob...

Previously, we reported that tasar silkworm pupal oil (TPO) is a rich source of alpha-linolenic acid (ALA) (37%). In this study, have used three chromatographic techniques, namely preparative HPLC, gas chromatography-mass spectroscopy (GC-MS), and chromatography flame ionization detection (GC-FID), to purify, determine, quantify (ALA), respectively, from TPO. Additionally, determine the potenti...

Mogroside V is one of the characteristic and effective components luohanguo extract, a food additive used as sweetener in Japan per Japan’s Standards Specifications for Food Additives (JSFA; 9th ed.). JSFA stipulates that quantitative determination mogroside content extract applies HPLC using analytical standard V. However, no reagents with proven purities are commercially available. Therefore ...

This paper describes two rapid, sensitive and specific methods for the determination of fulvestrant in pharmaceutical preparations by high performance liquid chromatography (HPLC) and linear sweep voltammetry (LSV). HPLC method was used to study the degradation behaviour. Fulvestrant was subjected to degradation under the conditions of hydrolysis (acid and alkali), oxidation (30% H2O2). The lin...

Voriconazole levels were determined with high-performance liquid chromatography (HPLC) and a microbiological agar diffusion assay using a Candida parapsilosis isolate in 103 serum samples from an HPLC-tested external quality control program (n = 39), 21 patients receiving voriconazole monotherapy (n = 39), and 7 patients receiving combination therapy (n = 25). The results of the bioassay were c...