BACKGROUND Central Middlesex Hospital, in northwest London, has screened neonates for hemoglobinopathies, using the established manual technique of isoelectric focusing (IEF) since 1989. Recently, this laboratory has faced a large increase in the number of samples tested per year. This study compared the detection of hemoglobin abnormalities between the existing manual IEF method and that of au...

oligosaccharide prepared from Pl.-B was eluted as a single peak on both reversed-phase {RP-) HPLC and size-fractionation (SF-) HPLC, and its strueture was estimated to be Manoct-qManal -. 3)(Xylfi1-.2) Manfi1-4GlcNAcfi1-4(Fuc=1-3)GlcNAc by a combination of component analysis, successive exogl)'cosidase digestions, IS-MS anal)'sis, and 500MHz 'H-NMR, Two tryptic gl)'copeptides were isolated from...

Filtration is a common method of sample preparation in dissolution testing, prior to HPLC injection. The purpose of sample filtration is to remove non-dissolved solids prior to HPLC injection. Non-dissolved solids interfere with the resulting chromatography by continuing to dissolve throughout the period of the analysis and also by plugging the HPLC column. It has already been shown that sample...

Cyanobacteria produce a wide range of potent toxins, including hepatotoxic microcystins. HPLC methods for microcystin analysis and purification almost invariably include acetonitrile in the elution gradient mobile phase. The recent, acute, global acetonitrile shortage requires that adequate methods are available for microcystin analysis and purification without the need for acetonitrile. Here w...

The qualitative and quantitative analysis of active constituents in Fructus Psoraleae is presented by high-performance liquid chromatography (HPLC) coupled with different detections. Extracts of Fructus Psoraleae were examined by HPLC with ion trap mass spectrometry (IT-MS) and 18 major compounds of coumarins, benzofuran glycosides, flavonoids, and meroterpene were identified. The determination...

A sensitive (0.01-1 nmol) method has been developed for the analysis of polyamines in higher plant extracts based on high performance liquid chromatography (HPLC) of their benzoyl derivatives (Redmond, Tseng 1979 J Chromatogr 170: 479-481). Putrescine, cadaverine, agmatine, spermidine, spermine, and the less common polyamines nor-spermidine and homospermidine can be completely resolved by rever...

SUMMARY The development of robust high-performance liquid chromatography (HPLC) technologies continues to improve the detailed analysis and sequencing of glycan structures released from glycoproteins. Here, we present a database (GlycoBase) and analytical tool (autoGU) to assist the interpretation and assignment of HPLC-glycan profiles. GlycoBase is a relational database which contains the HPLC...

A convenient method for the separation of molecular species of complex lipids such as sphingomyelin and ceramide by argentation high performance liquid chromatography (HPLC) is described for the first time. Molecular species separation of these complex lipids was achieved for quantitative analysis and preparative work by the combined use of argentation and reversed-phase HPLC.-Smith, M., P. Mon...

Using solid-phase extraction columns and "high-performance" liquid-chromatographic (HPLC) analysis, we could determine cyclosporin A and nine of its metabolites in blood, bile, and urine. To facilitate calculations of concentrations of cyclosporin A and its metabolites from the chromatograms, we used cyclosporin D as internal standard. For the HPLC analysis we used two sequential 250-mm analyti...

HPLC coupled simultaneously to NMR and MS is particularly suited to the identification of unknown polar, non-volatile compounds in complex environmental samples, i.e. to non-target analysis of such samples, if pollutants are present in the ppb range. Both methods give complementary structural information. Optimum information on unknowns is gained if both techniques are coupled on-line. This rev...