Integrative conjugative elements (ICEs) enable horizontal gene transfer among bacteria. In Pseudomonas, only a phenotypically distinct subpopulation of ICE-bearing cells can mobilize ICE DNA to new hosts. Transfer competence is a terminal state; division is limited, and many cells lyse.

A broad swath of eukaryotic microbial biodiversity cannot be cultivated in the lab and is therefore inaccessible to conventional genome-wide comparative methods. One promising approach to study these lineages is single cell genomics (SCG), whereby an individual cell is captured from nature and genome data are produced from the amplified total DNA. Here we tested the efficacy of SCG to generate ...

Conjugation and natural competence are two major mechanisms that explain the acquisition of foreign genes throughout bacterial evolution. In recent decades, several studies in model organisms have revealed in great detail the steps involved in such processes. The findings support the idea that the major basis of these mechanisms is essentially similar in all bacteria. However, recent work has p...

in this paper, it has been shown that there is a special relation between horizontal and vertical gradients of gravity data and the vertical and horizontal components of magnetic data for some of 2-d sources. it has been shown that the hilbert transform is useful in calculating the vertical gradient of gravity and magnetic anomalies from the horizontal gradient for transformation of gravity and...

for the characteristics of horizontal fractures in shallow low-permeability oil layers after hydraulic fracturing in multilayer reservoirs, horizontal fractures are taken equivalent to an elliptical cylinder with the reservoir thickness using the equivalent permeability model; then, upon the elliptic seepage theory, the seepage field which has led by a vertical well with horizontal fractures is...