X-ray CT is a powerful technology to study root growth in soil in-situ. Root systems can be studied in its true 3D geometry over time. Hence, the same plant can be scanned multiple times during development. A downside is the potential of X-rays to interfere with biological processes and therefore plant growth. The aim of this study is to evaluate the influence of cumulative X-ray dose on Vicia ...

Aleurone layers of Hordeum vulgare, cv. ;Himalaya' took up [(14)C]-abscisic acid (ABA) when incubated for various times. Radioactivity accumulated with time in a low speed, DNA-containing pellet accounting for 1.6 to 2.3% of the radioactivity recovered in subcellular fractions at 18 hours. Thin layer chromatography of ethanolic or methanolic extracts of the cytosol, which contained greater than...

Jörg Durner and Peter Böger Lehrstuhl für Physiologie und Biochemie der Pflanzen, Universität Konstanz, D-7750 Konstanz. Bundesrepublik Deutschland Z. Naturforsch. 43c, 850 -856 (1988); received July 18, 1988 Acetolactate Synthase (ALS), Acetohydroxy Acid Synthase (AHAS). Amino Acids (BranchedChain), Hordeum vulgare L., Feedback Inhibition Acetolactate synthase (EC 4.1.3.18; ALS) has been extra...

The levels of diversity and extent of linkage disequilibrium in cultivated species are largely determined by diversity in their wild progenitors. We report a comparison of nucleotide sequence diversity in wild and cultivated barley (Hordeum vulgare ssp. spontaneum and ssp. vulgare) at 7 nuclear loci totaling 9296bp, using sequence from Hordeum bulbosum to infer the ancestral state of mutations....

In plants the vacuolar functions are the cellular storage of soluble carbohydrates, organic acids, inorganic ions and toxic compounds. Transporters and channels located in the vacuolar membrane, the tonoplast, are modulated by PTMs to facilitate the optimal functioning of a large number of metabolic pathways. Here we present a phosphoproteomic approach for the identification of in vivo phosphor...

230 Journal of Plant Registrations, Vol 2, No. 3, September 2008 Devastating yield losses occurred in wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) in the western United States within 2 yr after the fi rst appearance of the Russian wheat aphid [RWA; Diuraphis noxia, (Mordvilko)] in 1986 (Porter et al., 1999). Screening of the entire USDA-ARS National Small Grains Collection of H....

Barley (Hordeum vulgare) is an economically important species, which can be cultivated in environmentally adverse conditions due to its higher tolerance in contrast to other cereal crops. The draft of H. vulgare genome is available already for couple of years; however its functional annotation is still incomplete. All available databases were searched to expand current annotation. The improved ...

Within the cereal aleurone reserve proteins are stored in specialized organelles, the protein storage vacuoles (PSV). We developed an aqueous method for the isolation of intact PSV. Barley (Hordeum vulgare L. cv Himalaya) aleurone protoplasts were gently lysed by passing them through a syringe needle. PSV were separated from cytoplasmic components by microfiltration and low-speed centrifugation...

Soil salinity is the major abiotic stress in plant agriculture strongly influencing productivity worldwide and it delimit the crop yield. Several abiotic stresses are united by the fact that at least part of their detrimental effect on plant performance is caused by disruption of plant water status. This can occur through decreased availability of water in the environment during drought, altere...