I . The plasma concentration of retinol-binding holoprotein (holo-RBP) in rats given retinol-sufficient diets containing 50 g soya-bean protein/kg was 90 yo of the concentration found in controls given zoo g soya-bean protein/kg, whereas in rats given 50 g rice protein/kg it was only 68 % of the control value. Plasma albumin concentrations in the low-protein groups were lower by 20-25 %. 2. The...

We investigated the co-ordinate variations of the two cobalamin (Cbl)-dependent enzymes, methionine synthase (MS) and methylmalonyl-CoA mutase (MCM), and measured the levels of their respective cofactors, methylcobalamin (CH3Cbl) and adenosylcobalamin (AdoCbl) in cultured human glioma cells during nitrous oxide exposure and during a subsequent recovery period of culture in a nitrous oxide-free ...

We have previously shown that (1) an acute deficiency in blood serum holo-ceruloplasmin (Cp) developed in rats that were fed fodder containing silver ions (Ag-fodder) for one month and (2) the deficiency in holo-Cp was compensated by non-hepatic holo-Cp synthesis in rats that were chronically fed Ag-fodder for 6 months (Ag-rats). The purpose of the present study is to identify the organ(s) that...

A heme-dependent conformational rearrangement of the C-terminal domain of heme binding protein (PhuS) is required for interaction with the iron-regulated heme oxygenase (HemO). Herein, we further investigate the underlying mechanism of this conformational rearrangement and its implications for heme transfer via site-directed mutagenesis, resonance Raman (RR), hydrogen-deuterium exchange MS (HDX...

IscR, an Fe-S cluster-containing transcriptional factor, regulates genes involved in various cellular processes. In response to environmental stimuli such as oxidative stress and iron levels, IscR switches between its holo and apo forms to regulate various targets. IscR binding sequences are classified into two types: the type 1 IscR box that is specific for holo-IscR binding, and the type 2 Is...

In biological fluids, hCG and its free alpha- (hCG alpha) and beta-subunits (hCG beta), occur in multiple forms. These various forms differ at the molecular level primarily in glycosylation, but also differ in protein backbone modifications corresponding to the urinary low molecular weight fragment of the hCG beta-subunit (beta-core fragment). This microheterogeneous nature can be demonstrated ...

Clustering high-dimensional data is a challenging task in data mining, and clustering high-dimensional categorical data is even more challenging because it is more difficult to measure the similarity between categorical objects. Most algorithms assume feature independence when computing similarity between data objects, or make use of computationally demanding techniques such as PCA for numerica...

PURPOSE To utilize MR to delineate the morphologic abnormalities of the temporal horn and adjacent structures in patients with congenital brain anomalies and to differentiate these findings from the temporal horn alterations in obstructive hydrocephalus. PATIENTS AND METHODS Thirty-six patients were included in this retrospective study, including eight with agenesis of the corpus callosum (AC...