Isolation and purification of biomolecules (doubleand singlestranded DNA, total RNAs, mRNAs, miRNA, proteins), as well as specific cells and organs, facilitate a wide array of downstream applications in gene and protein expression studies, cloning, transfection, protein-protein interactions, immunology, clinical diagnostics, cDNA library synthesis, PCR and qPCR, Sanger and NGS sequencing, and m...

embryonic stem cells are capable of differentiating to variety of cell tissues including cardiomyocytes. this developmental change is accompanied with a great deal of ion channel expression and functions. mouse stem cell derived cardiomyocytes were prepared and separated to yield isolated single cell suspension for cell current recording. in the present study some properties of the k + -current...

~~~~~~~~~~~~~~~~~~~~W Mr. N. SHAH, lecturer, Professorial Unit, the Institute of Laryngology and Otology, London W.C.1, writes: Myringotomy and aspiration of fluid from the middle ear is a common practice in otology. Children with fluid (serous or "glue ") are operated on under general anaesthesia with the aid of a Zeiss microscope. A glass suction cannula (see photograph) for aspiration of flu...

O mundo empresarial é repleto de situações complexas e inovadoras. Diante disso, muitos empresários buscam meios para se adequar às diversas normas impostas pela regulamentação civil comercial. Nesse contexto nasceu a necessidade criação uma empresa, que orientasse desse sequência aos objetivos da firma, como forma proteção dos bens conquistados no decorrer décadas. Para tanto, observando as ne...

TRPC5 channels are Ca(2+)-permeable nonselective cation channels activated by G-protein-coupled receptors, although the mechanisms responsible for channel activation and regulation are poorly understood. Carbachol-activated TRPC5 currents were recorded by the whole-cell patch clamp technique from human embryonic kidney 293 cells transiently transfected with TRPC5 and the M1 muscarinic receptor....

as the full test. It is performed as follows The apparatus.? (i) Small wooden racks about 12^ inclies by about 1^ inches (at the bottom) by 1^ inches, to hold 18 miniature test tubes, the bottom shelf painted black and the rest white, and the top numbered for the tubes. Each rack is also numbered, (w)' Durham's fermentation tubes measuring about 5 cm. by 8 mm. with internal diameter of 6 mm.A f...

To examine effects of cytosolic Na+, K+, and Cs+ on the voltage dependence of the Na+-K+ pump, we measured Na+-K+ pump current (Ip) of ventricular myocytes voltage-clamped at potentials (Vm) from 100 to +60 mV. Superfusates were designed to eliminate voltage dependence at extracellular pump sites. The cytosolic compartment of myocytes was perfused with patch pipette solutions with a Na+ concent...