OBJECTIVES Nicotine is a widely used drug of abuse exerting number of effects on the central nervous system. The study was aimed at the effects of nicotine in various doses on the excitability of cerebral cortex and - by using the methods of histochemistry - to identify possible signs of neuronal degeneration after nicotine administration. METHODS Cortical afterdischarges were elicited by rep...

Intrauterine growth of fetus mainly depends on adequate function of placenta. Placenta is a fetal organ. It shares same stress and strain to which the fetus is exposed. Thus any disease which affects the mother has a great impact on placenta. Anatomical structure of placenta greatly influences its function. Thus study of placental morphology is considered essential. 136 Pregnancy complications ...

In visual area V2 of monkeys, cytochrome oxidase (CO) histochemistry reveals a system of stripe-like subregions where densely labeled thick and thin stripes and pale interstripes can be recognized. Several lines of evidence suggest that CO stripe-like subregions are associated with functional streams in the visual cortex. In the present study, the distribution of retrogradely labeled callosal c...

In vivo imaging techniques can be integrated with classical histochemistry to create an actual histochemistry of water. In particular, Magnetic Resonance Imaging (MRI), an imaging technique primarily used as diagnostic tool in clinical/preclinical research, has excellent anatomical resolution, unlimited penetration depth and intrinsic soft tissue contrast. Thanks to the technological developmen...

In both non-ovulating and ovulating toads of the species, Bufo melanostictus, the fimbrium stained only weakly for mucopolysaccharides (MPs) whereas the infundibulum stained strongly for neutral MPs and also for glycogen. In the non-ovulating toad, only neutral and sulphated MPs were detected in the goblet cells of the upper magnum, whereas sulphated, neutral and sialomucins were detected in th...

Fructoaldolase activity in primate skin and appendages has been assayed with a highly sensitive fluorometric method. Epidermal fructoaldolase has characteristics similar to the fructoaldolases obtained from animal sources. The fructoaldolase activities of various skin structures are remarkably high; all glandular structures (except for apocrine sweat glands which have the highest activity of 24...

Collagen-based implants have been described as vehicles for transplantation of cultured skin cells for treatment of burn wounds. To optimize vascularization and repair of connective tissue, collagen solubility and glutaraldehyde crosslinking were evaluated. Cultured skin substitutes consisted of human keratinocytes and fibroblasts attached to collagen-glycosaminoglycan substrates that were prep...

The preparat ion of a specific antiserum (anti-CaBP) against the calcium-binding protein (CaBP) of the chorioaUantoic membrane ( C A M ) is described. The antiC a B P appeared to be specific for the C a B P by immunodiffusion and immunoelectrophoresis. Applicat ion of the ant i -CaBP in immunofluorescence histochemistry revealed that the C a B P is present in the C A M only at developmental age...

The complex carbohydrates in the bovine duodenal glands were examined histochemically using 5 peroxidase-labeled lectins to achieve a better knowledge of their glycoprotein profiles. The presence of at least 2 different cell types and secretion of neutral mucin were observed in the bovine duodenal glands. The duodenal gland cells located in the central area of the lobules contained neutral glyc...