BACKGROUND Pigeon circovirus (PiCV) is considered to be a viral agent central to the development of young pigeon disease syndrome (YPDS). The Cap protein, a structural protein encoded by the cap (or C1) gene of PiCV, has been shown to be responsible for not only capsid assembly, but also has been used as antigen for detecting antibody when the host is infected with PiCV. The antigenic character...

The authors recently noticed that the published amino acid sequence of the protein C epitope tag component of the CNAP tag was incorrect. The published amino acid sequence of the protein C (PC) epitope tag was EDQVDPIDGK and should have been EDQVDPRLIDGK. Thus, the correct amino acid sequence of the CNAP tag employed is: MEDQVDPRLIDGK-GGAGG-HHHHHHHHHH (protein C [PC] epitope tag is underlined, ...

While BIACORE instruments are routinely used for kinetic measurements and for the determination of binding constants, the immobilization of a ligand onto the sensor chip surface has to be individually optimized for every system. We show here that the histidine (His) tag, routinely used in protein purification and in detection is an ideal tag for immobilization, despite the intrinsically low aff...

His-tag affinity purification is one of the most commonly used methods to purify recombinant proteins expressed in E. coli. One drawback of using the His-tag is the co-purification of contaminating histidine-rich E. coli proteins. We engineered a new E. coli expression strain, LOBSTR (low background strain), which eliminates the most abundant contaminants. LOBSTR is derived from the E. coli BL2...