نتایج جستجو برای: his beloved woman

تعداد نتایج: 243129  

2012
Ralf Koetter

T HIS special issue is intended as a tribute to the work of Ralf Koetter. Ralf’s premature death on February 2, 2009 deprived our community not only of one of its most beloved members, but also of an imaginative individual contributor, valued collaborator, and respected leader who had already had a significant impact on coding theory and practice, and who could have potentially contributed so m...

2016

The Bristol Medico-Chirurgical Society mourns the loss of Lemuel Matthews Griffiths, who died on September 22nd, lri eightieth year, an Ex-President and one of its earliest an(i most active members. With this issue already in the Press it is quite impossible to do justice to his memory or give a(lequate expression to the well-earned gratitude of his medical c?nfreres in Bristol for the many yea...

2016
Ilaria Solano Pietro Parisse Ornella Cavalleri Federico Gramazio Loredana Casalis Maurizio Canepa

BACKGROUND A versatile strategy for protein-surface coupling in biochips exploits the affinity for polyhistidine of the nitrilotriacetic acid (NTA) group loaded with Ni(II). Methods based on optical reflectivity measurements such as spectroscopic ellipsometry (SE) allow for label-free, non-invasive monitoring of molecule adsorption/desorption at surfaces. RESULTS This paper describes a SE stu...

Journal: :The Biochemical journal 1999
C H Klaassen P H Bovee-Geurts G L Decaluwé W J DeGrip

Here we describe a generic procedure for the expression and purification of milligram quantities of functional recombinant eukaryotic integral membrane proteins, exemplified by hexahistidine-tagged bovine rhodopsin. These quantities were obtained with the recombinant baculovirus/Sf9 insect cell-based expression system in large-scale bioreactor cultures with the use of a serum-free and protein-f...

Journal: :Journal of molecular biology 2002
Markus Kaufmann Peter Lindner Annemarie Honegger Kerstin Blank Markus Tschopp Guido Capitani Andreas Plückthun Markus G Grütter

The crystal structure of a mutant form of the single-chain fragment (scFv), derived from the monoclonal anti-His tag antibody 3D5, in complex with a hexahistidine peptide has been determined at 2.7 A resolution. The peptide binds to a deep pocket formed at the interface of the variable domains of the light and the heavy chain, mainly through hydrophobic interaction to aromatic residues and hydr...

Journal: :The Biochemical journal 1993
H Klima A Klein G van Echten G Schwarzmann K Suzuki K Sandhoff

The cDNA of the human GM2-activator protein was cloned into the expression vector pHX17. The plasmid encodes a fusion protein with a hexahistidine tail and a Factor Xa cleavage site at its N-terminus. The recombinant protein was purified from cell homogenates under denaturing conditions by metal-ion affinity chromatography in a single step and then was refolded. The hexahistidine tail could be ...

2017
Dorothee Wasserberg Jordi Cabanas-Danés Jord Prangsma Shane O'Mahony Pierre-Andre Cazade Eldrich Tromp Christian Blum Damien Thompson Jurriaan Huskens Vinod Subramaniam Pascal Jonkheijm

We report oriented immobilization of proteins using the standard hexahistidine (His6)-Ni2+:NTA (nitrilotriacetic acid) methodology, which we systematically tuned to give control of surface coverage. Fluorescence microscopy and surface plasmon resonance measurements of self-assembled monolayers (SAMs) of red fluorescent proteins (TagRFP) showed that binding strength increased by 1 order of magni...

Journal: :Biochemistry 2006
David E Blair Omid Hekmat Alexander W Schüttelkopf Binesh Shrestha Ken Tokuyasu Stephen G Withers Daan M F van Aalten

The fungal pathogen Colletotrichum lindemuthianum secretes an endo-chitin de-N-acetylase (ClCDA) to modify exposed hyphal chitin during penetration and infection of plants. Although a significant amount of biochemical data is available on fungal chitin de-N-acetylases, no structural data exist. Here we describe the 1.8 A crystal structure of a ClCDA product complex and the analysis of the react...

Journal: :Chembiochem : a European journal of chemical biology 2009
Thomas André Annett Reichel Karl-Heinz Wiesmüller Robert Tampé Jacob Piehler Roland Brock

The development of synthetic, low-molecular-weight ligand receptor systems for the selective control of biomolecular interactions remains a major challenge. Binding of oligohistidine peptides to chelators containing Ni2+-loaded nitrilotriacetic acid (NTA) moieties is one of the most widely used and best-characterised recognition systems. Recognition units containing multiple NTA moieties (multi...

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