In late 1973 a slow release formulation of terbutryne (2-ethylamino-6-methylthio-4-tert.-butylamino-1,3,5triazine) was introduced commercially for the control of water weeds (CIBA-Geigy (Clarosan)). Hence it will be of increasing importance for residue analysts to be able to detect low concentrations of this compound in surface waters. In the work reported here two methods are described, one, h...

Isomangiferin was isolated from Cyclopia subternata using a multi-step process including extraction, liquid-liquid partitioning, high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase high-performance liquid chromatography (HPLC). Enrichment of phenolic compounds in a methanol extract of C. subternata leaves was conducted using liquid-liquid partitioning with ethy...

The tricyclic antidepressant group of drugs are amongst the most widely used medicaments in current clinical practice. They have proved to be extremely useful in the treatment of many different types of depression. There are, however, greater than ten-fold differences between different individual subjects in the rate at which they can degrade the various members of the group into biologically i...

The increasing demand for enantiopure drugs has led to the development of a variety of stereoselective separation technologies. Among them, high performance liquid chromatography (HPLC) is well recognized as a powerful, fast and efficient technique, which has been successfully employed for analysis and preparation of enantiomers of drugs. Nowadays, liquid chromatographic techniques are the focu...

The distribution of the neurotensin-related hexapeptide LANTG within the basal ganglia and its projection targets was studied in turtles, pigeons, and hamsters by using immunohistochemical techniques, radioimmunoassay (RIA), gel chromatography, and high performance liquid chromatography (HPLC).

Liquid column chromatography, planar chromatography (TLC) on modified and unmodified silica layers, reversed-phase high-pressure liquid chromatography (HPLC), as well as ESI-TOF MS and 1H-NMR have been used for separation, purification and identification of phenolic acids in the barks of Sambucus nigra and Viburnum opulus (Caprifoliaceae). By the use of these procedures three cinnamic acid deri...

Photonic crystal (PC) microcavities formed by 2D air holes on silicon-on-insulator substrates were utilized as biosensors to detect antibiotics with high specificity. The antibiotic, gentamicin, with molecular weight of only 478 g/mol, was successfully detected via its binding to corresponding antibody pre-immobilized on the sensor surface. Detection range (in concentration) was extended by com...

In this paper, analysis of free nucleotides from mouseliver tissue during different day times has been described. Perchloric acid extract of mouse liver tissue was neutralized with tri-N-octylamine in trichlorotriflouroethane and after removal of ClO4(-), subjected to preliminary purification on a Cu(2+)-loaded column of Chelex 100. A high-pressure liquid chromatographic (HPLC) anion-exchange p...

Glucosinolates are a well-studied and highly diverse class of natural plant compounds. They play important roles in plant resistance, rapeseed oil quality, food flavoring, and human health. The biological activity of glucosinolates is released upon tissue damage, when they are mixed with the enzyme myrosinase. This results in the formation of pungent and toxic breakdown products, such as isothi...