Employee resistance to change at State-Owned Enterprises (SOEs) is a visible phenomenon in both local and global contexts. Though it’s an expanding significant area, also heavily understudied, depicting literature empirical gaps the said scope. Under qualitative approach, case study strategy has been used explore employee Healthway General Hospital (HGH). In light of context, this emphasizes re...

Worldwide regulations during COVID-19 positively and negatively impacted self-management in paediatric patients with chronic medical conditions. We investigated the impact of on adherence to recombinant human growth hormone (r-hGH) therapy disorders, using real-world data extracted March 2019–February 2020 (before COVID-19) 2020–February 2021 (during from easypod™ connect ecosystem. Data three ...

Human growth hormone (hGH) signal transduction initiates with a receptor dimerization in which one molecule binds to the receptor through sites 1 and 2. A sandwich enzyme-linked immunosorbent assay was developed for quantifying hGH molecules that present helix 4 from binding site 1. For this, horse anti-rhGH antibodies were eluted by an immunoaffinity column constituted by sepharose-rhGH. These...

Gene activation in higher eukaryotes is often under the control of regulatory elements quite distant from their target promoters. It is unclear how such long-range control is mediated. Here we show that a single determinant of the human growth hormone locus control region (hGH LCR) located 14.5 kb 5prime prime or minute to the hGH-N promoter has a critical, specific, and nonredundant role in fa...

Only a few investigations concerning growth hormone (HGH) levels in maternal and cord blood in humans have been made. These were limited to normal pregnancies (Greenwood, Hunter, and Klopper, 1964; Yen, Pearson, and Stratman, 1965). Though the absolute values of HGH cannot be compared in these two investigations since different methods of measuring the HGH were used, both studies found a higher...

Human growth hormone (hGH) genomic sequence containing 5 exons and 4 introns was cloned in pcDNA-3 and the constructed plasmid was subsequently used for transfection ofNlli-3T3 cell line using lipofection technique. Expression of hGH in stably transfected cells was assayed using ELISA. Total RNA was extracted from transfected cells and hGH cDNA was amplified by RT-PCR using specific primers...

The effects of growth hormone (GH) treatment on renal sodium sulfate cotransport (NaSi-1) were studied in adult (9-10 months) and old (22-23 months) male Fischer 344 rats. All animals received twice-daily s.c. injections of recombinant human GH (hGH; 4 mg/kg) for up to 6 days. Animals were sacrificed by exsanguination on days 0, 1, 2, 3, 4, 5, and 6. Kidneys were removed, and kidney cortex was ...

plasma based on the solid-phase radioimmunoassay (RIA) using antibody-coated disposable microtiter trays was described. This method is simple, rapid and inexpensive,and particularly useful for ultramicro-measurement of plasma HGH levels in large numbers of plasma samples. The lower limit of sensitivity for purified HGH was 25pg/ml (P < 0.05). The precision of the measurement of HGH in plasma ha...

Glycophosphatidylinositol (GPI)-anchored membrane proteins are initially synthesized with a cleavable COOH-terminal extension that signals anchor attachment. Overexpression in COS cells of hGH-DAF fusion proteins containing the GPI signal of decay accelerating factor (DAF) fused to the COOH-terminus of human growth hormone (hGH), produces both GPI-anchored hGH-DAF and uncleaved precursors that ...

In this study, we examined human growth hormone (hGH)-induced changes in nonionic detergent solubility characteristics of its receptor (hGHR). Exposure of IM-9 cells to hGH caused a time- and concentration-dependent loss of immunoblottable detergent-extractable hGHRs and a corresponding accumulation of receptors in a detergent-insoluble pool. At 37°C, the loss of detergent-soluble and the accum...