A 1.85-kilobase HindIII nuclear DNA probe from Histoplasma capsulatum G217B detected polymorphic restriction fragments within whole-cell DNA from different clinical isolates of H. capsulatum, consistent with the previous system of classification. The probe failed to hybridize to DNA from Blastomyces dermatitidis, Candida spp., Saccharomyces cerevisiae, Sepedonium chrysospermum, and Chrysosporiu...

To the Editor: The last step in electrophoretic procedures for creatine kinase (CK; EC 2.7.3.2) isoenzyme separation on cellulose acetate is to incubate the plate with a reagent that will develop either fluorescence or color for each isoenzyme. In our laboratory we routinely use fluorescence to detect the isoenzymes. The reagent used must be highly sensitive, so that low proportions of the isoe...