Background and objective: Langerhans cell histiocytosis (LCH) is a rare histiocytic proliferative disorder of unknown etiology and mainly affects young children. The histological feature is granuloma-like proliferation of langerhans-type dendritic cells. Although the possible role of viruses such as Epstein-Barr virus (EBV, Human Herpes virus -4</...

A LOE FEROX AND WITHANIA SOMNIFERA are among southern African plants commonly used for the treatment of sexually transmitted infections (STIs). Aqueous extracts from both species, together with aloin, isolated from A. ferox, were evaluated for antiviral activity against herpes simplex virus type 1 (HSV-1) in vitro. The aqueous extracts showed detectable activity at a concentration of 1000 μg/ml...

Cerebellar disorders due to herpes simplex virus (HSV) infection are rare and always associated with herpes simplex encephalitis. We report 2 cases of severe primary acute cerebellitis caused by HSV type 1 that were identified by nested polymerase chain reaction performed on cerebrospinal fluid samples.

AMP was evaluated for its efficacy against herpes simplex virus type 2 genital infections in guinea pigs. Vaginally infected animals were treated twice a day with AMP at 50 mg/kg per dose for 2 weeks. Subcutaneous doses were started 10 weeks postinfection. AMP treatment did not reduce the number of animals with recrudescent episodes when compared with saline-treated controls. In addition, AMP t...

BHK-21 cells infected with temperature-sensitive mutants of herpes simplex virus type 1 strain KOS representing 16 complementation groups were tested for susceptibility to complement-mediated immune cytolysis at permissive (34 degrees C) and nonpermissive (39 degrees C) temperatures. Only cells infected by mutants in complementation group E were resistant to immune cytolysis in a temperature-se...

Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...

Herpes simplex virus type 1 was reactivated from the trigeminal ganglia of latently infected mice in a quantitative and time-dependent manner. Novobiocin and coumermycin A1 reversibly inhibited the reactivation of herpes simplex virus type 1. They did not inhibit viral replication in permissive cells (CV-1) but did inhibit replication in cells of neuronal origin (C1300) and acutely infected tri...

Rabbit eyes experimentally infected with either type 1 or type 2 herpes simplex viruses occasionally released virus spontaneously. Injection of adrenalin was not highly effective for stimulating virus release but did seem to have a slight and erratic activating capacity. No spontaneous virus release were detected from the eyes of six cats infected with cat herpesvirus, but, when adrenalin was a...

Subjects with oral herpes lesions at the time of serum sampling had higher-efficiency antibody (higher proportion of neutralizing antibody as determined by plaque reduction, compared with total antibody as detected by radioimmunoassay) to herpes simplex virus type 1 (HSV-1) than did subjects with no lesions at the time of serum sampling. These higher-efficiency sera also had higher antibody tit...

We have developed and tested a method for printing protein microarrays on glass slides and using these microarrays in a comparative fluorescence assay to detect TORCH infections. We generated protein microarrays by printing antibodies to simultaneously determine infections of TORCH (toxoplasmosis-rubella-cytomegalovirus-herpes) by detecting antibodies against Toxoplasma gondii, rubella virus, c...