For an understanding of the molecular basis of the marked decrease in catalase activity of various tumor cells, expression of the catalase gene was studied in rat and human hepatoma cell lines and in rat liver, which was used as a control with high activity. RNA blot hybridization profiles and run-on assays indicated that the decrease in catalase activity was due to depression of catalase gene ...

We have previously shown that BDS.1 rat hepatoma cells are hypersensitive to the antiproliferative effects of glucocorticoids, and secrete a glucocorticoid suppressible mitogenic activity (denoted GSM). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that GSM purified to near homogeneity migrated as a 28-kDa protein under nonreducing conditions and as a single 15-kDa polypept...

The ultrastructural aspect of liver invasion by Novikoff hepatoma transplanted in rat liver was investigated. Invasion of liver parenchyma was seen to begin by the projection of cytoplasmic processes from the tumor cells into the neighboring hepatic cells. This infiltration was, at times, associated with fragmentation of the invaded liver cells. Moreover, the tumor cells showed phagocytosis of ...

Previous studies have implicated phosphatidylethanolamine N-methyltransferase-2 (PEMT2) in the regulation of non-neoplastic liver growth [Tessitore,L., Cui,Z. and Vance,E. (1997) Biochem. J., 322, 151-154]. We have now investigated whether or not PEMT2 is also involved in the control of proliferation of hepatoma cells growing in an animal and cell death by apoptosis in the liver of tumor-bearin...

Sialic acid-binding immunoglobulin-like lectin-15 (Siglec-15) has been identified as a novel potential target for cancer immunotherapy. Here, we explored the role of Siglec-15 in human hepatoma cells. In this study, found that expression is substantially upregulated liver tissues comparison with nontumor tissues. Functionally, vitro experiments show promotes migration Furthermore, data demonstr...

A two-dimensional polyacrylamide gel electrophoresis technique is presented for separation of acid-extracted proteins of isolated nucleoli of normal rat liver and Novikoff hepatoma ascites cells. About 100 distinct protein spots were resolved. Comparison of the patterns for normal liver and Novikoff hepatoma revealed nine spots with markedly different intensities in the two patterns. Two spots ...

Received November 28,1979 Summary: Insulin causes a 5 to lo-fold increase in the velocity of a-aminoisobutyric acid transport and a 2 to 3-fold increase in tyrosine aminotransferase activity in dexamethasone-treated hepatoma tissue culture cells. Maximal responses occur 2-4 hours after insulin addition but then decrease to control levels by 24 hours incubation. Medium conditioned by cells incub...

BACKGROUND Limited knowledge about the molecular mechanism of avian influenza H9N2 virus pathogenicity in birds as well as human hosts has limited the development of effective control against the disease. To overcome this issue detailed understanding of the infectious characteristics of the virus in host cells should be obtained. OBJECTIVES In this study we examined the replication kinetics o...

SUMMARY A single injection of nitrosomethylurea (80 mg/kg body weight) to mice bearing hepatoma 22a led to an injury of the main components of the protein-synthesizing system (poly ribosomal complex and soluble factors of the cell sap) in liver and hepatoma cells. In liver cells of adult animals, the activity of the protein-synthesizing system returns to a normal level 5 to 15 hr after injectio...

Antinucleolar antisera were produced in rabbits immunized with whole isolated nucleoli from normal rat liver and rat Novikoff hepatoma ascites cells. These antisera produced positive nucleolar fluorescence of varying degrees in nucleoli and nuclei of Novikoff tumor, rat liver, kidney, and Walker tumor cells. Novikoff hepatoma and liver antinucleolar antisera fixed complement when combined with ...