Rat hepatoma tissue culture cells have retained only a few liver-specific membrane functions. To find out whether trans formation of these cells led to the appearance of tumor-specific membrane glycoprotein structures, which might also be pres ent in released form in the circulation of tumor-bearing animals, we compared ¡mmunologically the sera and ascites fluids of normal and of hepatoma-bear...

A study of kinetic properties of mitochondrial ATPase in Morris hepatoma 3924A is reported. The results show that submitochondrial particles isolated from the tumor tissue exhibited a three-fold increase in both the Km for ATP hydrolysis and Ki for the competitive inhibitor [beta, gamma-imido]ATP with regard to normal rat liver. Eadie-Hofstee analysis of the kinetics of ATP hydrolysis show that...

7-Hydroxy-6,8-dimethoxy-2H-1-benzopyran-2-one (isofraxidin) is a major coumarin component isolated from the stem bark of Acanthopanax senticosus, a widely used Chinese medicinal herb. We investigated isofraxidin in its anti-tumor effects on human hepatoma cell lines HuH-7 and HepG2. Isofraxidin significantly inhibited hepatoma cell invasion, without affecting cell attachment or growth. Expressi...

To elucidate the mechanism for hyperlipidemia in the hepatoma-bearing state, changes in some parameters related to the lipid metabolism and serum tumor necrosis factor-alpha (TNF-alpha) level were examined in Donryu rats that had been subcutaneously implanted with an ascites hepatoma cell line of AH109A. These parameters were also examined in rats that had been given a single injection of lipop...

We describe here a decrease in the ratio of phospholipids to protein in microsomes prepared from Morris hepatoma 7777, compared with those from host rat liver. These micro somes, characterized by their marker enzyme content, were found to have comparable enrichment of the NADPH cytochrome c reducÃ-ase. In addition to the decrease in phospholipid content, the major phospholipids had decreased po...

Thyroid hormone (T(3)) signaling through the thyroid hormone receptor (TRα1) regulates hepatoma cell growth and pathophysiology, but the underlying mechanisms are unclear at present. Here, we have shown that the oncomir microRNA-21 (miR-21) is activated by T(3) through a native T(3) response element in the primary miR-21 promoter. Overexpression of miR-21 promoted hepatoma cell migration and in...

Liver cancer is the second cause of death from cancer worldwide, without effective treatment. Traditional chemotherapy for liver cancer has big side effects for patients, whereas targeted drugs, such as sorafenib, commonly have drug resistance. Oroxylin A (OA) is the main bioactive flavonoids of Scutellariae radix, which has strong anti-hepatoma effect but low toxicity to normal tissue. To date...

DNA-RNA hybridization studies, using nuclear RNA's (nRNA's) labeled in vivo and in vitro with high specific radioactivities, were performed to compare the nRNA populations of normal rat liver, livers treated with 3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB), and 3'-Me-DAB-induced hepatomas. The study with normal liver nRNA labeled by i.p. injection of [3H]orotic acid indicated that the nucle...

Alpinetin is a type of novel plant flavonoid derived from Alpinia katsumadai Hayata, found to possess strong anti-hepatoma effects. However, the detailed antitumor mechanism of alpinetin remains unclear. Mitogen-activated protein kinase kinase-7 (MKK7) can regulate cellular growth, differentiation and apoptosis. The aim of this study was to investigate the role of MKK7 in the anti-hepatoma effe...