Mucopolysaccharides were extracted by Pronase diges tion from two types of rat tumor tissues: MC sarcoma in duced in Donryu rats by s.c. injection of methylcholanthrene and a solid tumor developed by s.c. transplantation of AH-109A cells into the animals. The mucopolysaccharides were fractionated by anion-exchange chromatography and characterized by chemical analysis, electrophoresis, infrared ...

The abilities of rodent tumor cell lines; B16BL6, ND and LT dietary variants of B16BL6, +SA, RT7-4bs and RT7-4bLs to invade composite collagen I gels containing heparin, chemically modified heparins, heparan sulfate, chondroitin sulfate, hyaluronic acid, dextran, dextran sulfate, laminin and collagen IV were investigated, and compared to the invasion of plain collagen I gels. The presence of he...

Monoclonal antibodies capable Of recognizing glycosaminoglycan (GAG) fine structures are likely to be of immense value in isolating and characterizing GAG chains with specific functional domains. We previously described the characterization of a monoclonal antibody directed against the chondroitin sulfate moiety of chick embryo fibroblast proteoglycan. This antibody is highly specific for GlcA(...

In recent years, progress in the fields of development and proteoglycan biology have produced converging evidence of the role of proteoglycans in morphogenesis. Numerous studies have demonstrated that proteoglycans are involved in several distinct morphogenetic pathways upon which they act at different levels. In particular, proteoglycans can determine the generation of morphogen gradients and ...

The abilities of rodent tumor cell lines; B16BL6, ND and LT dietary variants of B16BL6, +SA, RT7-4bs and RT7-4bLs to invade composite collagen I gels containing heparin, chemically modified heparins, heparan sulfate, chondroitin sulfate, hyaluronic acid, dextran, dextran sulfate, laminili and collagen IV were investigated, and compared to the invasion of plain collagen I gels. The presence of h...

Heparan sulfate proteoglycans are important modulators of growth factor signaling in a variety of patterning processes. Secreted growth factors that play critical roles in angiogenesis bind to heparan sulfate, and this association is affected by 6-O-sulfation of the heparan sulfate chains. Addition of 6-O-sulfate is catalyzed by a family of sulfotransferases (HS6STs), and genetic manipulation o...

Hemodynamic stress is a critical factor in the onset of atherosclerosis such that reduced rates of shear stress occurring at regions of high curvature are more prone to disease. The level of shear stress has direct influence on the thickness and integrity of the glycocalyx layer. Here we show that heparan sulfate, the main component of the glycocalyx layer, forms an intact layer only on cell su...