the objective was to investigate the presence of mycoplasma and chlamydia in the synovial fluid of patients with rheumatoid arthritis (ra) and other chronic arthritides. samples of synovial fluid (sf) were collected from all patients presenting with an articular effusion. seventy sf samples were subjected to study for mycoplasma and cultured on standard media for mycoplasma. the 70 other sf sam...

Mycoplasma contamination of cultured cell lines is difficult to detect by routine observation. Infected cells can display normal morphology and the slow growth rate of mycoplasma can delay detection for extended periods of time, compromising experimental results. Positive identification of mycoplasma typically requires cells to be either fixed and stained for DNA or processed with PCR. We prese...

mycoplasma genitalium (m.genitalium) is a sexually transmitted pathogen. detection of this microorganism in clinical specimens by culture is rather difficult and time consuming. the aim of this study was to produce polyclonal antibody against a synthetic peptide from p110 protein of m.genitalium in order to develop a diagnostic tool for detection of this microorganism in clinical specimens. a s...

It has been suggested that infection by some mycoplasma species can act as possible cofactors in the acceleration of immunodeficiency in HIV-infected patients. The present study was designed to examine infections by oral mycoplasma species in HIV-seropositive (HIV(+)) patients. Mycoplasma salivarium and Mycoplasma orale were isolated from 59.5% and 16.7% of 42 HIV(+) patients, respectively. Non...

Mycoplasma 352 Molecular characterization of mycoplasma gallisepticum isolated from chicken and turkey. 353 Pathogenicity of Mycoplasma gallisepticum strains using ELD50 in embryonated chicken eggs. 354 Evaluation of three DNA extraction methods for the detection of Mycoplasma spp. with an MG/MS multiplex real-time PCR method. 356 Use of MG/MS serology and PCR to determine flock status. 357 Exp...

One theory suggests that illnesses in Gulf War veterans may result from infection with Mycoplasma—bacteria-like microorganisms that are the smallest freeliving microorganisms. This chapter considers the Mycoplasma theory. It first discusses briefly the Mycoplasma species, including how they affect healthy persons and those with compromised immune systems. It then discusses hypotheses regarding ...

BACKGROUND Mycoplasma hyorhinis infection has been postulated to play a role in the development of several types of cancer, but the direct evidence and mechanism remained to be determined. METHODS Immunohistochemistry assay and nested polymerase-chain reaction (PCR) were performed to examine the mycoplasma hyorhinis infection in gastric cancer tissues. Statistical analysis was used to check t...