BACKGROUND Hypoxia seems to be an influencing factor for oral squamous cell carcinomas (SCC), and several immunohistochemical markers have been discussed in this regard. The aim of the present study was to evaluate preoperative hemoglobin levels as a prognostic factor for oral SCC. MATERIALS AND METHODS The files of 287 patients who had been treated for oral SCC between 1999 and 2008 were stu...

A recombinant hemoglobin containing Val beta 7 (Hb beta E7V) was engineered and expressed in yeast to evaluate amino acid specificity of the Glu beta 6-->Val mutation (Hb beta E6V) in promoting polymer formation of deoxyhemoglobin. The purified CO Hb beta E7V migrated as a single band on electrophoresis with a slightly decreased positive charge compared with CO Hb S. The oxygen affinity of Hb b...

OBJECTIVES The clinical analysis of hemoglobin by ion exchange chromatography can result in ambiguities in identification of the nature of the globin chain present in patient samples. LC/ESI-MS provides rapid and precise determination of globin chain masses. DESIGN AND METHODS Hemolysate of hemoglobin Q-India and hemoglobin S/D/F have been analyzed using ESI-MS. Tandem-MS has been used to est...

It has been known for many years that high levels of fetal hemoglobin (Hb F) have an important clinical benefit in patients with sickle cell anemia (SCA). This knowledge was initially based on the observation that populations of Indian and Arabian SCA patients with high Hb F levels have a milder clinical form of the disease.1 Later on, epidemiological studies demonstrated that patients with Hb ...

purpose: in this study the effect of iron supplmentation on hemoglobin and hematocrit in pregnant women with hb>13.2 g/dl was investigated. material and methods: in this experimental clincal study, the effect of iron supplementation on hemoglobin and hematocrit in pregnant women with hb>13.2 g/dl and ferritin> 14.3 μg/l during the 13th- 18th pregnancy week was studied. eighty seven women havin...

Background: High maternal hemoglobin (Hb) concentration was considered a risk factor for the developing of pregnancy hypertension. The purpose of this study was to determine whether high maternal Hb concentration in first trimesters associated with pregnancy induced hypertension in Iranian nulliparous women. Methods: This study was carried out in teaching hospital in Sari, Iran. Samples were ob...

This new approach to measurement of glycated hemoglobin (Hb A1) is a three-step procedure involving (a) conversion of oxyhemoglobin into NO-hemoglobin by sodium dithionite and sodium nitrite in the presence of inositol hexaphosphate; (b) incubation with haptoglobin, which preferentially binds glycated hemoglobin; and (c) determination of the hemoglobin/haptoglobin complex by its peroxidase acti...

Enhancing the stability and sensitivity of electrochemical biosensors is highly significant for their practical application. Herein, inspired by formation mussel foot protein, we proposed a strategy to construct hemoglobin-based biosensor hydrogen peroxide detection using hydrophobic ionic liquid (HIL) coordination assisted microfluidic technology. The active layer HIL@Hb was achieved mixing BB...

T HE HUMAN HEMOGLOBINS consist of two pairs of peptide chains.1’2 Adult hemoglobin ( Hb-A ) has two chains designated a and two chains designated iB; it is thus represented as 2A $2A#{149}Fetal hemoglobin (Hb-F ) has two a chains, which are similar if not identical in structure to those of Hb-A, but a pair of chemically distinct chains, the y chains replacing the $ chains.3’4 Hb-F is thus repre...

Factors contributing to the clinical differences between sickle cell-hemoglobin C disease (SC) and the benign sickle cell trait (AS) include the higher proportion of hemoglobin (Hb) S and the higher cell Hb concentrations in SC compared with AS red cells. Reports differ, however, about whether Hb C copolymerizes more than Hb A with Hb S when measured by minimum gelling concentrations (MGCs) and...