Introducción: La tifosis aviar y la pulorosis, enfermedades ocasionadas por Salmonella enterica subsp. serotipo Gallinarum biotipo Pullorum respectivamente, son responsables de una elevada mortalidad en aves corral generando grandes pérdidas económicas para los avicultores. Objetivo: Fortalecer detección agentes causales pulorosis mediante optimización técnicas moleculares, tales como PCR punto...

Background: DNA amplification method has been developed for identifying and discriminating Salmonella serovars, using specific primers at the genus and serovar levels and to identify the S. Enteritidis, S. Dublin, S. Gallinarum and S. Pullorum. Objectives: This study was conducted for molecular identification and discrimination among some important Salmonella serovars. Methods: Fifty isolates o...

New Zealand is free from all the major epidemic (Office International des Epizooties List A) diseases of animals and other important diseases, such as rabies and the transmissible spongiform encephalopathies. The once endemic conditions of sheep scab (Psoroptes ovis), bovine brucellosis (Brucella abortus), hydatids (Echinococcus granulosus) and Aujeszky's disease have been eradicated. Anthrax (...

Heparinized whole blood, parasitized with Aegyptianella pullorum, was collected from 2 fowls. Buffered lactose peptone (BLP) was added v/v as a stabilizer and the mixture lyophilized in 2 ml aliquots after rapid or slow freezing. At different stages during the freeze-drying process, as well as after lyophilization and reconstitution with 1.8 ml of sterile water, samples were taken and injected ...

ABSTRACT: Pullorum disease is described worldwide and caused by Salmonella enterica subspecies serovar Gallinarum biovar (S. Pullorum). S. infection important in commercial poultry, provoking a systemic with high mortality rates. Its occurrence requires notification, when it diagnosed breeding flocks, its eradication demanded. The aim of this study was to report severe outbreak young Guinea fow...