Apolipoprotein (apo) B-100, the protein constituent of low density lipoproteins (LDL), is the determinant responsible for LDL binding to the apoB,E(LDL) receptor on cells. The current study was designed to identify the region(s) of apoB-100 that interact with the apoB,E(LDL) receptor. Apolipoprotein B-100 was fragmented by thrombin digestion, and the isolated fragments (T2, T3, T4) were recombi...

OBJECTIVE Higher selenium level has been hypothesized to have the potential to reduce the risk of cardiovascular diseases including dyslipidemia. However, results from previous studies are inconsistent. This study aims to determine the association between selenium level and dyslipidemia in elderly Chinese with relatively low selenium status. METHODS A cross-sectional study of 1859 participant...

We have developed a procedure for labeling lipoproteins with the fluorescent probe 3,3'-dioctadecylindocarbocyanine (Dil) and have used Dil-labeled native and acetoacetylated lipoproteins to differentiate macrophages from fibroblasts in mixed cell culture. Lipoproteins labeled with this probe were suitable for the direct viewing of their binding and internalization by cells in vitro. The labeli...

BACKGROUND Hypertension is one of the main comorbidities associated with dyslipidemia. This study aimed to examine the extent to which dyslipidemia increases the risk of developing hypertension in a Japanese working-age male population. METHODS AND RESULTS We analyzed data from 14 215 nonhypertensive male workers (age 38±9 years) who underwent annual medical checkups. Subjects were followed u...

BACKGROUND Many studies showed a moderate cholesterol-lowering effect of plant sterols (PS), but increased circulating PS might be atherogenic. We evaluated the associations between natural dietary intake of PS and carotid intima-media thickness (IMT) and serum lipids. METHODOLOGY/PRINCIPAL FINDINGS This community-based cross-sectional study included 1160 men and 2780 women aged 31-75 years. ...

BACKGROUND Several prospective epidemiologic studies have demonstrated that high-sensitivity C-reactive protein (hsCRP) is an effective serum marker for cardiac risk assessment. When hsCRP is considered in conjunction with traditional lipid screening, its clinical utility is further increased. In this report, hsCRP, HDL-cholesterol (HDLC), and total cholesterol (TC) assay imprecision is evaluat...

OBJECTIVE Lowering the blood concentration of low-density lipoprotein (LDL) cholesterol is the primary strategy employed in treating atherosclerotic disorders; however, most commonly prescribed statins prevent cardiovascular events in just 30% to 40% of treated patients. Therefore, additional treatment is required for patients in whom statins have been ineffective. In this study of atherosclero...

OBJECTIVE Assessing the prevalence of cardiometabolic factors in residents from a community in the north of Valencia city, Venezuela, and establishing clustering patterns for the cardiometabolic alterations. METHODS One hundred people aged 19 to 77 were studied. Weight, height, abdominal circumference, blood pressure, glucose, lipid profile and insulin were all measured. Insulin resistance wa...

Hamsters are increasingly used for comparative study of cholesterol metabolism (1). To assess the validity of differential precipitation with Mg2 4-phosphotungstate for quantification of plasma high-density-lipoprotein cholesterol (HDLC), we did a method-comparison with ultracentrifugal flotation. The precipitating reagent, 0.55 mmolJL phosphotungstic acid and 25 mmol/L magnesium chloride, was ...

[Purpose] The present study investigated the effects of regular exercise on the improvement of free fatty acid (FFA) levels and metabolic risk factors of stroke patients. [Methods] The subjects were 20 male patients aged 47-59 years who were diagnosed as having hemiplegia resulting from stroke. Exercise was conducted using a stationary bicycle, a rehabilitative exercise machine. The exercise pr...