The antisickling effect of dried Aristeus antennatus was investigated to ascertain the efficacy extract sample inhibit polymerization sickle cell haemoglobin (HbSS), and improve Fe2+/Fe3+ ratio blood. first dried, ground into powder soaked in chloroform defat it order produce fat soluble fraction (filtrate). defatted residue air-dried later methanol for 24 hours obtain fraction. fractions (FAS)...

The antisickling properties of crude juice extracts of the edible portions of three commonly consumed tropical fruits namely Persia americana, Citrus sinensis, and Carica papaya were investigated in vitro alongside a new drug preparation called Ciklavit® that has antisickling activity. Four different solvent extracts of the crude juice of each fruit including aqueous, acidic, alkaline and alcoh...

In this protocol, gene expression in yeast (Saccharomyces cerevisiae) is changed after exposure to oxidative stress induced by the addition of hydrogen peroxide (H₂O₂), an oxidizing agent. In the experiment, yeast is grown for 48 hours in 1/2X YPD broth containing 3X glucose. The culture is split into a control and treated group. The experiment culture is treated with 0.5 mM H₂O₂ in Hanks Buffe...

A method for the selective recovery of living microfilariae from Onchocerca volvulus nodules is described. The microfilariae migrate through solidified agar gel into overlayering Hanks balanced salt solution (HBSS). The highest recovery rates of the worms were obtained with 0.3 and 0.4% agar. Optimal conditions for in vitro cultures of the larvae in HBSS were established; pH range 7.0-7.5, gluc...

The mechanism of protein S-nitrosation in cells is not fully understood. Using rat 3Y1 cells, we addressed this issue. Among S-nitrosothiols and NO donors tested, only S-nitrosocysteine (CysNO) induced S-nitrosation when exposed in Hanks' balanced salt solution (HBSS) and not in serum-containing general culture medium. In HBSS, NO release from CysNO was almost completely abolished by sequesteri...

Aim: To evaluate the cytotoxicity of several storage media (coconut water, whole milk, isotonic solution, Hank ́s Balanced Salt Solution and tap water) using a multiparametric cytotoxicity analysis employing 3T3 cells. Methods: Plates containing confluent 3T3 fibroblasts were exposed to the various media for 24h, at 37°C with 5% CO2, and cell viability was evaluated by a multiparametric assay as...

Objective: This study aims to develop and validate a simple, rapid accurate HPLC method for the determination of rosmarinic acid (RA), an active marker Thunbergia laurifolia (TL) tea, in Hank's Balanced Salt Solution (HBSS) using HPLC.
 Methods: The separation was performed Xterra® C18 reversed-phase column (150 3.9 mm, 5 µm). mobile phase consisted 0.5% (v/v) glacial acetic (A) acetonitri...