Free radical formation in heme proteins is recognized as a factor in mediating the toxicity of many chemicals. The present study was designed to evaluate the dose-response relationship of the free radical scavenging properties of pentoxifylline in nitri te-induced Hb oxidation. Different concentrations of pentoxifylline were added at different time intervals of Hb oxidation in erythrocytes lysa...

A procedure is described for visualization of cells that had only fetal hemoglobin apnormal and mutant adult hemoglobins in peared as clear ghosts. Analyses of cord human blood smears. After extraction of blood from newborn infants indicate that, blood smears with a concentrated poalthough most erythrocytes contain only Hb tassium phosphate buffer (2.76 M, pH 7.2), F and a few contain only Hb A...

Staphylococcus aureus is a common and serious cause of infection in humans. The bacterium expresses a cell-surface receptor that binds to, and strips haem from, human haemoglobin (Hb). The binding interface has previously been identified; however, the structural changes that promote haem release from haemoglobin were unknown. Here, the structure of the receptor-Hb complex is reported at 2.6 Å r...

It has been found possible to detect the presence of some gamma chain abnormal fetal hemoglobins in adults through analysis of genomic DNA with selected restriction endonucleases. These variants are Hb F-Hull (A gamma 121Glu----Lys) which was observed in eight adult members of five families, Hb F-Pendergrass (A gamma 36Pro----Arg) in five adult members of one family, and Hb F-Port Royal (G gamm...

BACKGROUND The literature regarding the nonspecificity of applications of the Jaffe (alkaline picrate) reaction for creatinine is generally outdated. We conducted a specificity study to update the nonspecificity information for current Jaffe and enzymatic creatinine assays. METHODS Two serum pools with creatinine concentrations within the pediatric reference interval were spiked with albumin,...

A new turbidimetric method for the direct measurement of the solubility of oxy- and deoxyhemoglobins (Hb) in concentrated phosphate buffer has been established. The principle of the method is the formation of a homogeneous emulsion when hemoglobin is introduced in concentrated phosphate buffer. The solubility of the oxy and deoxy forms of Hb A, Hb S, Hb C, Hb F, and Hb CHarlem (beta 6Glu leads ...

Because of the potential for methemoglobinemia during nitric oxide therapy in newborns, methods are needed to accurately quantify methemoglobin (MetHb) in the presence of the high concentrations of fetal hemoglobin (Hb F), bilirubin, and lipids seen in these patients. Spectral differences between fetal and adult Hbs invalidate assumptions of conventional multiwavelength Hb photometry, so we eva...

T HE HUMAN HEMOGLOBINS consist of two pairs of peptide chains.1’2 Adult hemoglobin ( Hb-A ) has two chains designated a and two chains designated iB; it is thus represented as 2A $2A#{149}Fetal hemoglobin (Hb-F ) has two a chains, which are similar if not identical in structure to those of Hb-A, but a pair of chemically distinct chains, the y chains replacing the $ chains.3’4 Hb-F is thus repre...

Isoelectric focusing (IEF) of hemoglobin was compared to the classical chromatography of labeled globin chains for 22 antenatal diagnoses of hemoglobinopathies: 1 1 for /9 thalassemia. and 1 1 for sickle cell disease. In all cases. the two methods gave identical results. The diagnosis was confirmed after birth or abortion. Three fetuses homozygous for /1 thalassemia and one homozygous for sickl...

Introduction: Ficus capensis has been used in traditional medicine to treat anaemia, tuberculosis, convulsion, pains, wounds, respiratory disorders, and other health challenges. This study investigated the effect of F. ethanolic leaf extract phenylhydrazine (PHZ)-induced anaemia Wistar rats. Methods: Induction was done by intraperitoneal administration PHZ (40 mg/kg for 48 hours). A normal grou...