Mg(OH)2 conversion coatings were formed on an Mg-2Zn-0.5Mn-Ca-Ce alloy via hydrothermal method at three different synthesizing temperatures (160, 170 and 180 ̋C). The effect of synthesizing temperature on microstructure and electrochemical property of the coatings were systematically studied. With increasing synthesizing temperature, the coating became thicker due to the faster reaction and dep...

The mechanism of protein S-nitrosation in cells is not fully understood. Using rat 3Y1 cells, we addressed this issue. Among S-nitrosothiols and NO donors tested, only S-nitrosocysteine (CysNO) induced S-nitrosation when exposed in Hanks' balanced salt solution (HBSS) and not in serum-containing general culture medium. In HBSS, NO release from CysNO was almost completely abolished by sequesteri...

Nonrhegmatogenous retinal detachments were formed in the eyes of Dutch rabbits by subretinal injection of Hanks' balanced salt solution. The electroretinogram (ERG) was recorded locally from the acutely detached retina, and simultaneously from the surrounding attached retina (vitreal ERG [VERG]), before and after exposure to diffuse intense irradiation. Light adaptation elevated b-wave threshol...

Brief formalin fixation in the cold prior to histochemical assay of rat liver and pancreas for various dehydrogenases has been used successfully to circumvent the structural damage and enzymatic loss to which mitochondria of frozen sections would otherwise be subject. To obtain an optimal result a single set of conditions has been devised, including fixation prior to freezing of minute (finely ...

Investigations were carried out on 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX), a potent direct-acting bacterial mutagen found in chlorinated tap water, for the potential to induce 6-thioguanine (6-TG) resistant mutations in cultured Chinese hamster V79 cells. The mutagenicity of MX was manifested when cells were treated with MX in Hanks balanced salt solution, but not in serum-fr...

38 Computer The Cooler the Better: New Directions in the Nomadic Age T hanks to rapid progress in microelectronics technology, a new, nomadic lifestyle has become widespread these days. People, regardless of location, enjoy greater connectivity through communication networks and intelligent electronic terminals. This nomadic lifestyle will become even more common as technology frees people from...

We studied the role of the retinal pigment epithelium (RPE) in the resorption of different subretinal fluids from under small experimental retinal detachments (blebs) in the rabbit. Damaging the RPE with sodium iodate caused the resorption time, for blebs made with an ionic solution (Hanks'), to decrease from 2-6 hours to only about 30 minutes. Blebs made with sucrose also absorbed much more qu...

To better understand the mechanism of leukocyte migration in complex environments, model extracellular matrices were prepared using gelatin, Hanks' solution, Bodipy-BSA (fluorescent upon proteolysis), and dihydrotetramethylrosamine or hydroethidine (fluorescent upon oxidation). Using quantitative microfluorometry, neutrophil-mediated extracellular pulses of reactive oxygen metabolites (ROMs) an...

Monochloramine (NH2Cl) is known to be one of the virulence factors in Helicobacter pylori-associated gastric mucosal injury. The present study was designed to examine NH2Cl-evoked DNA fragmentation in the gastric epithelial cell line MKN45. NH2Cl was produced by mixing NH3 with sodium hypochlorite (NaClO). MKN45 cells were exposed to NH2Cl, NH3, or NaClO in Hanks' balanced salt solution. DNA cl...