The production of sesquiterpene-type phytoalexins with a vetispyradiene skeleton by Hyoscyamus albus hairy roots induced by methyl jasmonate (MeJA) was reported in a previous paper. The production pattern on co-treatment with cupper sulfate and MeJA (CuSO(4)-MeJA) showed a TLC profile differing from that on treatment with MeJA. Thus, we studied the production of phytoalexins on hairy root cultu...

Hairy root culture is a promising approach to improve production of plant secondary metabolites. The genes, which are located in T-DNA root-inducing plasmid, regulate auxin sensitivity hairy roots. Therefore, this study was aimed the growth and rosmarinic acid Lavandula angustifolia Lateral branches roots were transferred ½ MS B5 liquid media. To assess sensitivity, indole-3-acetic (IAA) indole...

The plant Picrorhiza kurroa Royle ex. Benth is a fast depleting medicinal value plant. This plant yields a crystalline product called ‘‘kutkin,’’ in its root, which is usually a mixture of two major C9-iridoid glycosides, i.e., picroside-I (6-Otrans-cinnamoyl catalpol) and kutkoside (10-O-vaniloylcatalpol). The increasing market demand of this phytoconstituent has led to threat of extinction of...

Here, we present the draft genome of Rhizobium rhizogenes strain ATCC 15834. The genome contains 7,070,307 bp in 43 scaffolds. R. rhizogenes, also known as Agrobacterium rhizogenes, is a plant pathogen that causes hairy root disease. This hairy root induction has been used in biotechnology for the generation of transgenic root cultures.

The development of an efficient protocol for successful hairy root induction by Agrobacterium rhizogenes is the key step toward an in vitro culturing method for the mass production of secondary metabolites. The selection of an effective Agrobacterium strain for the production of hairy roots is highly plant species dependent and must be determined empirically. Therefore, our goal was to investig...

Hairy root cultures were obtained from diploid and induced tetraploid plants of Datura stramonium and analyzed by gas chromatography/mass spectrometry. Twenty alkaloids (19 for diploid and 9 for tetraploid hairy root cultures) were identified. A new tropane ester 3-tigloyloxy-6-propionyloxy-7-hydroxytropane was identified on the basis of mass spectral data. Hyoscyamine was the main alkaloid in ...

In vitro raised shoots of Plumbago rosea L. were infected with A4 strain of Agrobacterium rhizogenes to initiate hairy root formation, which produced 3.0±0.33 hairy roots per incision on explants in 20 d incubation. Southern blot analysis confirmed the integration of T-DNA into the genome of the roots. The hairy roots were cultured on MS agar medium supplemented with 2.0 mg/L BAP to induce the ...

Turnip (Brassica rapa ssp. rapa) is an important vegetable crop producing glucosinolates (GSLs) and phenolic compounds. The GSLs, phenolic compound contents and transcript levels in hairy root cultures, as well as their antioxidant, antimicrobial and anticancer activity were studied in turnip. Transgenic hairy root lines were confirmed by polymerase chain reaction (PCR) and reverse transcriptio...

Hairy root culture (HRC) represent a valuable biotechnological tool for the production of plant secondary metabolites. Secondary metabolome study Arabidopsis thaliana may help to understand biological roles various metabolites present in it. The work deals with establishment Agrobacterium rhizogenes strains transformed HRC A. very high transformation frequency resulting long term hairy cultures...