Forty Pasteurella multocida subsp. multocida strains collected from cattle with haemorrhagic septicaemia belonging to serotype B:2, 5 and two reference strains of serotype B:2, were examined. The strains were isolated in the years 1958 to 1962 and 1985 to 1995. Analysis of the polimorfism of restricted DNA fragments by the use of the pulsed-field gel electrophoresis (PFGE) technique of contour-...

A total of 3,257 samples of diseased rainbow trout were examined for the presence of viruses from January 1983 to December 1987. A virus closely related to the VR 299 serotype of infectious pancreatic necrosis virus was isolated from 13 cases. An additional 7,228 viscera samples from asymptomatic fish were collected during the same period and a similar virus was isolated from 2 sites. During th...

The in vitro influence of methisoprinol on the replication of viral haemorrhagic septicaemia (VHS) and infectious haematopoetic necrosis (IHN) viruses by measuring their RNA synthesis was examined. EPC cell cultures containing various concentrations of methisoprinol (0, 100, 200, 300, 400 and 500 μg/ml of medium) were inoculated with VHS or IHN virus suspension containing 10 TCID50/ml. The inhi...

Pasteurella multocida (Robert type-1) was grown on tryptose sucrose yeast (TSY) broth in a fermenter. A sterilised air was provided that potentiated the density of the culture (5 x 10 organisms/ml). The culture was inactivated with formalin (0.5% ). The alum precipitated and oil based haemorrhagic septicaemia vaccines (APHSV and OBHSV) were prepared and their efficacies were evaluated in rabbit...

Viral haemorrhagic septicaemia virus (VHSV) genotype IVb was recently detected as the cause of numerous mortality events in Great Lakes fish. In situ hybridization was used to examine the gonads from 13 fish, including freshwater drum Aplodinotus grunniens and muskellunge Esox masquinongy that were infected naturally, as well as rainbow trout Oncorhynchus mykiss and fathead minnows Pimphales pr...

A commercial plant extract derived from olive tree leaf (Olea europaea) (LExt) and its major compound, oleuropein (Ole), inhibited the in vitro infectivity of the viral haemorrhagic septicaemia virus (VHSV), a salmonid rhabdovirus. Incubation of virus with LExt or Ole before infection reduced the viral infectivity to 10 and 30%, respectively. Furthermore, LExt drastically decreased VHSV titers ...